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AB171344

Human IL-20 ELISA Kit

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(1 Publication)

Human IL-20 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-20 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

ZCYTO10, UNQ852/PRO1801, IL20, Interleukin-20, IL-20, Cytokine Zcyto10

1 Images
Sandwich ELISA - Human IL-20 ELISA Kit (AB171344)
  • sELISA

Unknown

Sandwich ELISA - Human IL-20 ELISA Kit (AB171344)

Representative standard curve using ab171344.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

62.5 - 4000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human IL-20 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human IL-20 in cell culture supernatants, serum and plasma (heparin, EDTA).

An IL-20 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for IL-20 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human IL-20 amount of sample captured in plate.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab171344-1x96Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB171344-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB171344-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB171344-CMP08", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Seal", "number":"AB171344-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB171344-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB171344-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB171344-CMP04", "productcode":"" }, { "size":"2 x 10 ng", "name":"Lyophilized recombinant Human IL-20 standard", "number":"AB171344-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL-20 antibody Microplate (12 x 8 wells)", "number":"AB171344-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated anti-Human IL-20 antibody", "number":"AB171344-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB171344-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Pro-inflammatory and angiogenic cytokine mainly secreted by monocytes and skin keratinocytes that plays crucial roles in immune responses, regulation of inflammatory responses, hemopoiesis, as well as epidermal cell and keratinocyte differentiation (PubMed : 17277128, PubMed : 34403503). Enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity (PubMed : 17277128). Affects multiple actin-mediated functions in activated neutrophils leading to inhibition of phagocytosis, granule exocytosis, and migration (PubMed : 28424238). Exert its effects via the type I IL-20 receptor complex consisting of IL20RA and IL20RB (PubMed : 11706020). Alternatively, can mediate its activity through a second receptor complex called type II IL-20 receptor complex composed of IL22RA1 and IL20RB (PubMed : 11564763). Acts as an arteriogenic and vascular remodeling factory by activating a range of signaling processes including phosphorylations of JAK2 and STAT5 as well as activation of the serine and threonine kinases AKT and ERK1/2 (By similarity). Alternatively, can activate STAT3 phosphorylation and transcriptional activity in a JAK2, ERK1/2 and p38 MAPK-dependent manner in keratinocytes (PubMed : 23614738).
See full target information IL20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 9:795 PubMed29719541

2018

A Reappraisal on the Potential Ability of Human Neutrophils to Express and Produce IL-17 Family Members : Failure to Reproducibly Detect It.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Tamassia,Fabio Arruda-Silva,Federica Calzetti,Silvia Lonardi,Sara Gasperini,Elisa Gardiman,Francisco Bianchetto-Aguilera,Luisa Benerini Gatta,Giampiero Girolomoni,Alberto Mantovani,William Vermi,Marco A Cassatella
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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