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AB231927

Human IL-20 ELISA Kit

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(1 Publication)

Human IL-20 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-20 with a sensitivity of 4.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ZCYTO10, UNQ852/PRO1801, IL20, Interleukin-20, IL-20, Cytokine Zcyto10

4 Images
Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)
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Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)

Human IL-20 Competitor standard curve comparison.

Standard curve comparison between Human IL-20 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)
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Supplier Data

Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)

Interpolated concentrations of spiked IL-20 in human serum and plasma samples.

The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Serum from ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). No detectable levels of IL-20 were measured.

Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)

Interpolated concentrations of spiked IL-20 in human cell culture media sample.

The concentrations of IL-20 were measured in duplicates, interpolated from the IL-20 standard curves and corrected for sample dilution. Undiluted samples are as follows : media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-20 ELISA Kit (AB231927)

Example of human IL-20 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.2 pg/mL

Range

7.8 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-20 ELISA Kit (ab231927) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-20 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-20 with 4.2 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "102 - 112 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "89 - 97 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "91 - 103 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "96 - 107 %", "average": "= 98" }, { "sample": "Cell culture media", "range": "105 - 111 %", "average": "= 109" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231927-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231927-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231927-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231927-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231927-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231927-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-20 Capture Antibody", "number":"AB231927-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-20 Lyophilized Recombinant Protein", "number":"AB231927-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB231927-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-20 Detector Antibody", "number":"AB231927-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231927-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Pro-inflammatory and angiogenic cytokine mainly secreted by monocytes and skin keratinocytes that plays crucial roles in immune responses, regulation of inflammatory responses, hemopoiesis, as well as epidermal cell and keratinocyte differentiation (PubMed : 17277128, PubMed : 34403503). Enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity (PubMed : 17277128). Affects multiple actin-mediated functions in activated neutrophils leading to inhibition of phagocytosis, granule exocytosis, and migration (PubMed : 28424238). Exert its effects via the type I IL-20 receptor complex consisting of IL20RA and IL20RB (PubMed : 11706020). Alternatively, can mediate its activity through a second receptor complex called type II IL-20 receptor complex composed of IL22RA1 and IL20RB (PubMed : 11564763). Acts as an arteriogenic and vascular remodeling factory by activating a range of signaling processes including phosphorylations of JAK2 and STAT5 as well as activation of the serine and threonine kinases AKT and ERK1/2 (By similarity). Alternatively, can activate STAT3 phosphorylation and transcriptional activity in a JAK2, ERK1/2 and p38 MAPK-dependent manner in keratinocytes (PubMed : 23614738).
See full target information IL20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 132: PubMed36006737

2022

IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfei He,Wenqian Luo,Yingjie Liu,Yuan Wang,Chengxin Ma,Qiuyao Wu,Pu Tian,Dasa He,Zhenchang Jia,Xianzhe Lv,Yu-Shui Ma,Haitang Yang,Ke Xu,Xue Zhang,Yansen Xiao,Peiyuan Zhang,Yajun Liang,Da Fu,Feng Yao,Guohong Hu
View all publications
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websiteProtocolBooklet
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