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AB314366

Human IL-20R2 ELISA Kit

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Human IL-20R2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-20R2 with a sensitivity of 49.592 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

DIRS1, UNQ557/PRO1114, IL20RB, Interleukin-20 receptor subunit beta, IL-20 receptor subunit beta, IL-20R-beta, IL-20RB, Fibronectin type III domain containing 6, IL-20R2, FNDC6

2 Images
Sandwich ELISA - Human IL-20R2 ELISA Kit (AB314366)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-20R2 ELISA Kit (AB314366)

Interpolated concentration of native IL-20R2 was measured in duplicate at different sample concentrations. Undiluted samples are 1,000 µg/mL HL-60 extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human IL-20R2 ELISA Kit (AB314366)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-20R2 ELISA Kit (AB314366)

Example of human IL-20R2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 49.592 pg/mL

Range

281.25 - 18000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB282017

Anti-IL-20R2 antibody [EPR24558-81] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-IL-20R2 antibody [EPR24558-81] - BSA and Azide free (AB282017)

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  • 0
Primary Antibodies

AB5984

Anti-IL-22RA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-IL-22RA1 antibody (AB5984)

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  • 4
Proteins & Peptides

AB152009

Recombinant Human IL-20R1 protein

  • 0
  • 0
Proteins & Peptides

AB191969

Recombinant Human IL-20R2 protein (Fc Chimera)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-20R2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-20R2 protein in Cell Lysate. Quantitate Human IL-20R2 with 49.592 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "100 - 103 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314366-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB314366-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB314366-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB314366-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB314366-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB314366-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-20R2 Lyophilized Recombinant Protein", "number":"AB314366-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-20R2 Capture Antibody", "number":"AB314366-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-20R2 Detector Antibody", "number":"AB314366-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB314366-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB314366-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB314366-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The IL20RA/IL20RB dimer is a receptor for IL19, IL20 and IL24. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.
See full target information IL20RB
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com