AB119542
Human IL-21 ELISA Kit is a sandwich ELISA designed to quantify Human IL-21 with a sensitivity of 20 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 78 - 5000 pg/mL
View Alternative Names
Interleukin-21, IL-21, Za11, IL21
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human IL-21 ELISA Kit (AB119542)
Typical Standard Curves.
Representative Standard Curve using ab119542.
Reactivity data
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Product details
Human IL-21 ELISA Kit ab119542 is a sandwich ELISA to measure Human IL-21 in serum, plasma, cell culture supernatant with a sensitivity of 20 pg/ml.
How the assay works
IL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human IL-21 ELISA Kit ab119542 protocol summary
1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately
How the assay works
IL-21 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-21 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-21 captured on the plate.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human IL-21 ELISA Kit ab119542 protocol summary
1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately
Precision
[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]
Recovery
[ { "sample": "Cell culture supernatant", "range": null, "average": "= 95" }, { "sample": "Serum", "range": null, "average": "= 71" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (PubMed : 11081504, PubMed : 15178704). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity).
Publications (7)
Recent publications for all applications. Explore the full list and refine your search
Scientific reports 14:7290 PubMed38538704
2024
A novel proteomic signature of osteoclast differentiation unveils the deubiquitinase UCHL1 as a necessary osteoclastogenic driver.
Applications
WB
Species
Mouse
Maria Materozzi,Massimo Resnati,Cecilia Facchi,Matteo Trudu,Ugo Orfanelli,Tommaso Perini,Luigi Gennari,Enrico Milan,Simone Cenci
International journal of molecular sciences 24: PubMed37762039
2023
Single-Cell RNA-Sequencing Reveals Peripheral T Helper Cells Promoting the Development of IgG4-Related Disease by Enhancing B Cell Activation and Differentiation.
Applications
Unspecified application
Species
Unspecified reactive species
Zongfei Ji,Weiqi Lu,Sifan Wu,Yong Zhang,Dan Meng,Xiao Zhang,Xiaojuan Dai,Huiyong Chen,Lili Ma,Ying Sun,Lindi Jiang,Xiufang Kong
Journal of immunology research 2022:9003902 PubMed36339942
2022
The Regulation between CD4CXCR5 Follicular Helper T (Tfh) Cells and CD19CD24CD38 Regulatory B (Breg) Cells in Gastric Cancer.
Applications
Unspecified application
Species
Unspecified reactive species
Ying Zhang,Jun Wu,Haibo Zhang,Chen Wu
Bioengineered 12:310-324 PubMed33446013
2021
MicroRNA-146a-5p enhances T helper 17 cell differentiation via decreasing a disintegrin and metalloprotease 17 level in primary sjögren's syndrome.
Applications
Unspecified application
Species
Unspecified reactive species
Xiaoyan Wang,Shaojun Xin,Youqing Wang,Duo Ju,Quan Wu,Ye Qiu,Xuemin Niu,Wei Liu,Jianyou Li,Pengtian Ji
International journal of medical sciences 17:3065-3072 PubMed33173427
2020
IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway.
Applications
Unspecified application
Species
Unspecified reactive species
Zongtao Liu,Yixuan Wang,Jiawei Shi,Si Chen,Li Xu,Fei Li,Nianguo Dong
Frontiers in pediatrics 6:139 PubMed29977885
2018
Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155.
Applications
Unspecified application
Species
Unspecified reactive species
Zhen Zhang,Xinlun Dai,Ji Qi,Yu Ao,Chunfeng Yang,Yumei Li
Modern rheumatology 28:1004-1008 PubMed29385874
2018
Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment.
Applications
Unspecified application
Species
Unspecified reactive species
Motohisa Yamamoto,Kenichi Takano,Ryuta Kamekura,Chisako Suzuki,Shingo Ichimiya,Tetsuo Himi,Hiroshi Nakase,Hiroki Takahashi
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