AB216170

Human IL-22 ELISA Kit

Recombinant
SimpleStep
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(4 Publications)

Human IL-22 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-22 with a sensitivity of 2.61 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

ILTIF, ZCYTO18, UNQ3099/PRO10096, IL22, Interleukin-22, IL-22, Cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor, IL-TIF

5 Images

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Interpolated concentrations of spiked IL-22 in PBMC cell culture supernatant sample.

The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1015 pg/mL in PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

Conditioned media was harvested after 48 hours. IL-22 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-22 were measured in duplicate, interpolated from the IL-22 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 316 pg/mL in PHA stimulated PBMC supernatant and 93 pg/mL in unstimulated supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Interpolated concentrations of spike IL-22 in human serum, plasma and urine samples.

The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1174 pg/mL in serum, 1099 pg/mL in plasma (citrate), 1197 pg/mL in plasma (heparin), 1189 pg/mL in plasma (EDTA) and 1,322 pg/mL in urine.

sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Example of human IL-22 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Example of human IL-22 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.61 pg/mL

Range

13.12 - 840 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-22 ELISA Kit (ab216170) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-22 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-22 with 2.61 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Serum", "range": "100 - 104 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "100 - 105 %", "average": "= 102" }, { "sample": "Urine", "range": "101 - 111 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "99 - 104 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "91 - 97 %", "average": "= 93" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed : 17204547). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed : 17204547). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB (PubMed : 10875937, PubMed : 18599299). Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways (PubMed : 25793261, PubMed : 31311100). Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (By similarity). Promotes epithelial cell spreading (By similarity).

See full target information IL22

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 27:106 PubMed38298427

2024

Microwave ablation enhances the systemic immune response in patients with lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fuqi Ma,Yuhua Lin,Zhenhua Ni,Shiqiang Wang,Mengjie Zhang,Xiaoe Wang,Zhuhua Zhang,Xuming Luo,Xiayi Miao

Experimental and therapeutic medicine 22:744 PubMed34055060

2021

miR-489-3p inhibits TLR4/NF-κB signaling to prevent inflammation in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujian Ye,Ping Wang,Fangmei Zhou

Experimental and therapeutic medicine 20:2148-2156 PubMed32765690

2020

IL-22/IL-22R1 promotes proliferation and collagen synthesis of MRC-5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Liu,Biao Shang,Jing Bai

The Journal of allergy and clinical immunology 144:1025-1035.e7 PubMed31251950

2019

Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces T2/T17 polarization through dectin-2.

Applications

Unspecified application

Species

Unspecified reactive species

Yunsheng Liang,Bihui Yu,Junchen Chen,Haijin Wu,Yingping Xu,Bin Yang,Qianjin Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-22 ELISA Kit

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Sandwich ELISA - Human IL-22 ELISA Kit (AB216170)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (4)

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