Human IL-22 ELISA Kit, Chemiluminescent

• sELISA

Lab

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• sELISA

Lab

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

Standard curve comparison between the Chemiluminescent Human IL-22 SimpleStep ELISA and colorimetric Human IL-22 SimpleStep ELISA (ab216170) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

• sELISA

Lab

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

Interpolated concentration of native IL-22 was measured in duplicate at different sample concentrations. Undiluted samples are 50% PHA-M (1.5% for 48 hours) stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was measured in the 50% unstimulated PBMC cell culture supernatant samples (data not shown).

• sELISA

Lab

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

Dynamic range comparison between the Chemiluminescent Human IL-22 SimpleStep ELISA, colorimetric Human IL-22 SimpleStep ELISA (ab216170), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

• sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

Conditioned media was harvested after 48 hours. IL-22 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-22 were measured in duplicate interpolated from the IL-22 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IL-22 concentration was determined to be 316 pg/mL in PHA stimulated PBMC supernatant and 93 pg/mL in unstimulated supernatant.

• sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

Interpolated concentrations of spike IL-22 in human serum plasma and urine samples.

The concentrations of IL-22 were measured in duplicates interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50% plasma (citrate) 50% plasma (heparin) 50% plasma (EDTA) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean spiked IL-22 concentration was determined to be 1174 pg/mL in serum 1099 pg/mL in plasma (citrate) 1197 pg/mL in plasma (heparin) 1189 pg/mL in plasma (EDTA) and 1322 pg/mL in urine.

• sELISA

Supplier Data

Sandwich ELISA - Human IL-22 ELISA Kit, Chemiluminescent (AB326388)

Interpolated concentrations of spiked IL-22 in PBMC cell culture supernatant sample.

The concentrations of IL-22 were measured in duplicates interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean spiked IL-22 concentration was determined to be 1015 pg/mL in PBMC supernatant.