Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-23 in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Images
Publications
Filter by
- Dental materials : official publication of the Academy of Dental Materials 39:204-2162023Immunomodulatory IL-23 receptor antagonist peptide nanocoatings for implant soft tissue healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn A Pizarek,Nicholas G Fischer,Conrado AparicioPubMed 36642687
- Disease markers 2021:40294702021Therapeutic Effects of 25-Hydroxyvitamin D on the Pathological Process of Benign Prostatic Hyperplasia: An In Vitro Evidence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanbo Chen,Hui Xu,Chong Liu,Meng Gu,Qi Chen,Ming Zhan,Zhong WangPubMed 34671434
- International journal of molecular sciences 20:2019Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaria Shishkova et. al.PubMed 31731607
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.1 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 15.6 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed:11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed:29287995, PubMed:32474165, PubMed:33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed:12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed:32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed:17676044).
Alternative names
SGRF, UNQ2498/PRO5798, IL23A, Interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19
What's included?
Recommended products
Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-23 in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.1 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate (12 x 8 well strips)
- Sensitivity
- = 15.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 113 | Range 108 - 120 % |
Sample type EDTA Plasma | Average % = 113 | Range 110 - 116 % |
Sample type Cell culture media | Average % = 118 | Range 114 - 124 % |
Sample type Heparin Plasma | Average % = 83 | Range 80 - 86 % |
Sample type Citrate plasma | Average % = 107 | Range 103 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-23 ELISA Kit (ab221837) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-23 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-23 with 15.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-23 acts as a growth factor for activated T and NK cells. IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases. IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit). The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits.
The standard is affinity purified.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
ELISA - Human IL-23 ELISA Kit (ab221837)
Example of human IL-23 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human IL-23 ELISA Kit (ab221837)
Interpolated concentrations of spiked IL-23 in human serum and plasma samples.
The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
ELISA - Human IL-23 ELISA Kit (ab221837)
Interpolated concentrations of native IL-23 in human cell culture supernatant samples.
The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: DMSO and LPS stimulated THP-1 cell culture supernatant 100% and IFN-γ and LPS stimulated THP-1 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23 concentration was determined to be 346 pg/mL in DMSO and LPS stimulated THP-1 cell culture supernatant and 834 pg/mL in IFN-γ and LPS stimulated THP-1 cell culture supernatant. Media and unstimulated THP-1 cell culture supernatant were undetectable.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com