Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-23 in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Colorimetric
Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
39.1 - 2500 pg/mL
1h 30m
= 15.6 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed:11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed:29287995, PubMed:32474165, PubMed:33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed:12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed:32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed:17676044).
SGRF, UNQ2498/PRO5798, IL23A, UNQ2498/PRO5798, SGRF, Interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19
Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-23 in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Colorimetric
Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
39.1 - 2500 pg/mL
1h 30m
Microplate (12 x 8 well strips)
= 15.6 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 5 | mean - | SD - | C.V. 4 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.7 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 113 | Range 108 - 120 % |
Sample type EDTA Plasma | Average % = 113 | Range 110 - 116 % |
Sample type Cell culture media | Average % = 118 | Range 114 - 124 % |
Sample type Heparin Plasma | Average % = 83 | Range 80 - 86 % |
Sample type Citrate plasma | Average % = 107 | Range 103 - 110 % |
Blue Ice
+4°C
+4°C
+4°C
Human IL-23 ELISA Kit (ab221837) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-23 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-23 with 15.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-23 acts as a growth factor for activated T and NK cells. IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases. IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit). The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits.
The standard is affinity purified.
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Example of human IL-23 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of spiked IL-23 in human serum and plasma samples.
The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Interpolated concentrations of native IL-23 in human cell culture supernatant samples.
The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: DMSO and LPS stimulated THP-1 cell culture supernatant 100% and IFN-γ and LPS stimulated THP-1 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23 concentration was determined to be 346 pg/mL in DMSO and LPS stimulated THP-1 cell culture supernatant and 834 pg/mL in IFN-γ and LPS stimulated THP-1 cell culture supernatant. Media and unstimulated THP-1 cell culture supernatant were undetectable.
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