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Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-23 in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

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Images

ELISA - Human IL-23 ELISA Kit (AB221837), expandable thumbnail
  • ELISA - Human IL-23 ELISA Kit (AB221837), expandable thumbnail
  • ELISA - Human IL-23 ELISA Kit (AB221837), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

39.1 - 2500 pg/mL

Assay time

1h 30m

Sensitivity

= 15.6 pg/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed:11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed:29287995, PubMed:32474165, PubMed:33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed:12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed:32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed:17676044).

Alternative names

What's included?

1 x 96 Tests
Components
10X Human IL-23 Capture Antibody
1 x 600 µL
10X Human IL-23 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
Antibody Diluent 4BI
1 x 6 mL
Human IL-23 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-23 in Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

39.1 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

Sensitivity

= 15.6 pg/mL

Precision

Intra assay

Sample

Serum

n

5

mean

-

SD

-

C.V.

4

Inter assay

Sample

Serum

n

3

mean

-

SD

-

C.V.

2.7

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 113

Range

108 - 120 %

Sample type

EDTA Plasma

Average %

= 113

Range

110 - 116 %

Sample type

Cell culture media

Average %

= 118

Range

114 - 124 %

Sample type

Heparin Plasma

Average %

= 83

Range

80 - 86 %

Sample type

Citrate plasma

Average %

= 107

Range

103 - 110 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human IL-23 ELISA Kit (ab221837) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-23 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-23 with 15.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-23 acts as a growth factor for activated T and NK cells. IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases. IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit). The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits.

The standard is affinity purified.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • ELISA - Human IL-23 ELISA Kit (ab221837), expandable thumbnail

    ELISA - Human IL-23 ELISA Kit (ab221837)

    Example of human IL-23 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • ELISA - Human IL-23 ELISA Kit (ab221837), expandable thumbnail

    ELISA - Human IL-23 ELISA Kit (ab221837)

    Interpolated concentrations of spiked IL-23 in human serum and plasma samples.

    The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • ELISA - Human IL-23 ELISA Kit (ab221837), expandable thumbnail

    ELISA - Human IL-23 ELISA Kit (ab221837)

    Interpolated concentrations of native IL-23 in human cell culture supernatant samples.

    The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows: DMSO and LPS stimulated THP-1 cell culture supernatant 100% and IFN-γ and LPS stimulated THP-1 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23 concentration was determined to be 346 pg/mL in DMSO and LPS stimulated THP-1 cell culture supernatant and 834 pg/mL in IFN-γ and LPS stimulated THP-1 cell culture supernatant. Media and unstimulated THP-1 cell culture supernatant were undetectable.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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