AB221837

Human IL-23 ELISA Kit

Recombinant
SimpleStep
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(4 Publications )

Human IL-23 ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Human IL-23 with a sensitivity of 15.6 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327479

3 Images

Sandwich ELISA - Human IL-23 ELISA Kit (AB221837)

sELISA

Supplier Data

Sandwich ELISA - Human IL-23 ELISA Kit (AB221837)

Interpolated concentrations of native IL-23 in human cell culture supernatant samples.

The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows : DMSO and LPS stimulated THP-1 cell culture supernatant 100% and IFN-γ and LPS stimulated THP-1 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23 concentration was determined to be 346 pg/mL in DMSO and LPS stimulated THP-1 cell culture supernatant and 834 pg/mL in IFN-γ and LPS stimulated THP-1 cell culture supernatant. Media and unstimulated THP-1 cell culture supernatant were undetectable.

sELISA

Supplier Data

Sandwich ELISA - Human IL-23 ELISA Kit (AB221837)

Interpolated concentrations of spiked IL-23 in human serum and plasma samples.

The concentrations of IL-23 were measured in duplicates, interpolated from the IL-23 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

ELISA - Human IL-23 ELISA Kit (AB221837)

ELISA

Supplier Data

ELISA - Human IL-23 ELISA Kit (AB221837)

Example of human IL-23 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 15.6 pg/mL

Range

39.1 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-23 ELISA Kit (ab221837) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-23 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-23 with 15.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-23 acts as a growth factor for activated T and NK cells. IL-23 induces auto immune inflammation and may be responsible for auto immune inflammatory diseases. IL-23 is a complexed heterodimer of the IL-23A (19 subunit) and IL-12B (p40 subunit). The ab221837 pair recognize the heterodimer by using antibodies to both the p19 and p40 subunits.

The standard is affinity purified.

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Precision & recovery information

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

Recovery

[ { "sample": "Serum", "range": "108 - 120 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "110 - 116 %", "average": "= 113" }, { "sample": "Cell culture media", "range": "114 - 124 %", "average": "= 118" }, { "sample": "Heparin Plasma", "range": "80 - 86 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "103 - 110 %", "average": "= 107" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed : 11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed : 29287995, PubMed : 32474165, PubMed : 33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed : 12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed : 32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed : 17676044).

See full target information IL23A

Alternative Names

SGRF, UNQ2498/PRO5798, IL23A, Interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19

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Publications (4)

Recent publications for all applications. Explore the full listand refine your search

Frontiers in oncology 13:1218808 PubMed37456250

2023

Evaluation of the impacts of photodynamic therapy on the prognosis of patients with hrHPV infection based on BTNL8 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hongqing Lv,Shuai Lou,Lin Zhang,Dawei Cui,Yao Li,Ying Yang,Meilan Chen,Pan Chen

Dental materials : official publication of the Academy of Dental Materials 39:204-216 PubMed36642687

2023

Immunomodulatory IL-23 receptor antagonist peptide nanocoatings for implant soft tissue healing.

Applications

Unspecified application

Species

Unspecified reactive species

John A Pizarek,Nicholas G Fischer,Conrado Aparicio

Disease markers 2021:4029470 PubMed34671434

2021

Therapeutic Effects of 25-Hydroxyvitamin D on the Pathological Process of Benign Prostatic Hyperplasia: An In Vitro Evidence.

Applications

Unspecified application

Species

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Yanbo Chen,Hui Xu,Chong Liu,Meng Gu,Qi Chen,Ming Zhan,Zhong Wang

International journal of molecular sciences 20: PubMed31731607

2019

Calcium Phosphate Bions Cause Intimal Hyperplasia in Intact Aortas of Normolipidemic Rats through Endothelial Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Daria Shishkova,Elena Velikanova,Maxim Sinitsky,Anna Tsepokina,Olga Gruzdeva,Leo Bogdanov,Anton Kutikhin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-23 ELISA Kit

Sandwich ELISA - Human IL-23 ELISA Kit (AB221837)

Sandwich ELISA - Human IL-23 ELISA Kit (AB221837)

ELISA - Human IL-23 ELISA Kit (AB221837)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

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Reactivity data

Product details

Precision & recovery information

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Publications (4)

Product promise