Human IL-23 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-23 in Human in Plasma, Cell culture supernatant, Serum samples.
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- Medicine 102:e323842023Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui Yang,Youjiang QiuPubMed 36820569
- BMC complementary medicine and therapies 21:1612021Cryptolepine inhibits hepatocellular carcinoma growth through inhibiting interleukin-6/STAT3 signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeth A Domfeh,Patrick W Narkwa,Osbourne Quaye,Kwadwo A Kusi,Gordon A Awandare,Charles Ansah,Alimatu Salam,Mohamed MutocheluhPubMed 34078370
- PloS one 6:e286802011Intensive cytokine induction in pandemic H1N1 influenza virus infection accompanied by robust production of IL-10 and IL-6.
- Clinical and experimental immunology 156:405-122009Combination use of immune complexes and a Ca2(+) channel blocker azelnidipine enhances interleukin-12 p40 secretion without T helper type 17 cytokine secretion in human monocyte-derived dendritic cells.Applications:ELISAReactive species:Unspecified reactive speciesT Abe,I Fuse,M Narita,M Takahashi,Y AizawaPubMed 19438591
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156.25 - 5000 pg/mL
- Assay time
- 2h 45m
- Sensitivity
- < 20 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed:11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed:29287995, PubMed:32474165, PubMed:33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed:12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed:32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed:17676044).
Alternative names
SGRF, UNQ2498/PRO5798, IL23A, Interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19
What's included?
Recommended products
Human IL-23 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-23 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 156.25 - 5000 pg/mL
- Assay time
- 2h 45m
- Assay Platform
- Microplate
- Sensitivity
- < 20 pg/mL
- General recovery
- 89%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 6 | mean 4637 pg/mL | SD 72 | C.V. 1.6 |
Sample Sample B | n 6 | mean 3908 pg/mL | SD 36 | C.V. 1 |
Sample Sample C | n 6 | mean 3016 pg/mL | SD 72 | C.V. 2.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample A | n 9 | mean 4439 pg/mL | SD 276 | C.V. 6.2 |
Sample Sample B | n 9 | mean 3807 pg/mL | SD 353 | C.V. 9.2 |
Sample Sample C | n 9 | mean 2570 pg/mL | SD 388 | C.V. 15 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 118 | Range 103 - 134 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Abcam's IL-23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-23 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
A monoclonal antibody specific for IL-23 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-23 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-23 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-23 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-23.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human IL-23 ELISA Kit (ab64708)
Representative Standard Curve using ab64708
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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