AB64708

Human IL-23 ELISA Kit

Name: Human IL-23 ELISA Kit (ab64708) | Abcam
Brand: Abcam Limited
SKU: AB64708
Price: 760 USD
Availability: InStock

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(5 Publications)

Human IL-23 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-23 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

SGRF, UNQ2498/PRO5798, IL23A, Interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19

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Sandwich ELISA - Human IL-23 ELISA Kit (AB64708)

sELISA

Supplier Data

Sandwich ELISA - Human IL-23 ELISA Kit (AB64708)

Representative Standard Curve using ab64708

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

156.25 - 5000 pg/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-23 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-23 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

A monoclonal antibody specific for IL-23 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-23 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-23 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-23 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-23.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 9, "mean": "4439 pg/mL", "standardDeviation": "276", "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 9, "mean": "3807 pg/mL", "standardDeviation": "353", "coefficientOfVariability": "9.2" }, { "reproducibilityType": "Inter", "sample": "Sample C", "replicates": 9, "mean": "2570 pg/mL", "standardDeviation": "388", "coefficientOfVariability": "15" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 6, "mean": "4637 pg/mL", "standardDeviation": "72", "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 6, "mean": "3908 pg/mL", "standardDeviation": "36", "coefficientOfVariability": "1" }, { "reproducibilityType": "Intra", "sample": "Sample C", "replicates": 6, "mean": "3016 pg/mL", "standardDeviation": "72", "coefficientOfVariability": "2.4" } ]

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Recovery

[ { "sample": "Serum", "range": "103 - 134 %", "average": "= 118" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab64708-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB64708-CMP04" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB64708-CMP03" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB64708-CMP05" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB64708-CMP10" }, { "size":"1 x 1 Unit", "name":"IL-23 Microplate(12 x 8 well strips)", "number":"AB64708-CMP11" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB64708-CMP06" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB64708-CMP01" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB64708-CMP09" }, { "size":"2 x 1 Vial", "name":"IL-23 Standard (Lyophilized)", "number":"AB64708-CMP07" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB64708-CMP08" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-23", "number":"AB64708-CMP02" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab64708-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB64708-CMP04", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-23 Microplate(12 x 8 well strips)", "number":"AB64708-CMP11", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB64708-CMP06", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB64708-CMP01", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB64708-CMP10", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB64708-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB64708-CMP08", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB64708-CMP09", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-23 Standard (Lyophilized)", "number":"AB64708-CMP07", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB64708-CMP03", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-23", "number":"AB64708-CMP02", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed : 11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed : 29287995, PubMed : 32474165, PubMed : 33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed : 12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed : 32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed : 17676044).

See full target information IL23A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Medicine 102:e32384 PubMed36820569

2023

Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Gui Yang,Youjiang Qiu

Translational research : the journal of laboratory and clinical medicine 239:18-34 PubMed34380068

2021

Circulating Mir-140 and leptin improve the accuracy of the differential diagnosis between psoriatic arthritis and rheumatoid arthritis: a case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Cheleschi,Sara Tenti,Giorgio Bedogni,Antonella Fioravanti

BMC complementary medicine and therapies 21:161 PubMed34078370

2021

Cryptolepine inhibits hepatocellular carcinoma growth through inhibiting interleukin-6/STAT3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Seth A Domfeh,Patrick W Narkwa,Osbourne Quaye,Kwadwo A Kusi,Gordon A Awandare,Charles Ansah,Alimatu Salam,Mohamed Mutocheluh

PloS one 6:e28680 PubMed22174866

2011

Intensive cytokine induction in pandemic H1N1 influenza virus infection accompanied by robust production of IL-10 and IL-6.

Applications

ELISA

Species

Unspecified reactive species

Xuelian Yu,Xi Zhang,Baihui Zhao,Jiayu Wang,Zhaokui Zhu,Zheng Teng,Junjie Shao,Jiaren Shen,Ye Gao,Zhengan Yuan,Fan Wu

Clinical and experimental immunology 156:405-12 PubMed19438591

2009

Combination use of immune complexes and a Ca2(+) channel blocker azelnidipine enhances interleukin-12 p40 secretion without T helper type 17 cytokine secretion in human monocyte-derived dendritic cells.

Applications

ELISA

Species

Unspecified reactive species

T Abe,I Fuse,M Narita,M Takahashi,Y Aizawa

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-23 ELISA Kit

Sandwich ELISA - Human IL-23 ELISA Kit (AB64708)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

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