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AB267657

Human IL-23R ELISA Kit

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(1 Publication)

Human IL-23R ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-23R in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-23 receptor, IL-23 receptor, IL-23R, IL23R

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Sandwich ELISA - Human IL-23R ELISA Kit (AB267657)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-23R ELISA Kit (AB267657)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture media, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Range

0.205 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-23R ELISA Kit is designed for the quantitative determination of IL-23R in cell culture supernatants, plasma and serum samples.

ΔNote: Human IL-23 R concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for human IL-23R coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-23R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-23R antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-23R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "74 - 99 %", "average": "= 88.31" }, { "sample": "Plasma", "range": "68 - 90 %", "average": "= 75.54" }, { "sample": "Cell culture supernatant", "range": "69 - 80 %", "average": "= 74.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267657-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267657-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267657-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL-23R coated Microplate (12 x 8 wells)", "number":"AB267657-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-23R Standard (Lyophilized)", "number":"AB267657-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267657-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human IL-23R Detection Antibody", "number":"AB267657-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB267657-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267657-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB267657-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.
See full target information IL23R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 12:310-324 PubMed33446013

2021

MicroRNA-146a-5p enhances T helper 17 cell differentiation via decreasing a disintegrin and metalloprotease 17 level in primary sjögren's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Wang,Shaojun Xin,Youqing Wang,Duo Ju,Quan Wu,Ye Qiu,Xuemin Niu,Wei Liu,Jianyou Li,Pengtian Ji
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websiteProtocolBooklet
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