Human IL-23R ELISA Kit
- Recombinant
- SimpleStep
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Human IL-23R ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-23R with a sensitivity of 9.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Interleukin-23 receptor, IL-23 receptor, IL-23R, IL23R
- sELISA
Supplier Data
Sandwich ELISA - Human IL-23R ELISA Kit (AB277716)
Example of human IL-23R standard curve in Sample Diluent NS. The IL-23R standard curve was prepared as described in Section 10.
Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human IL-23R ELISA Kit (AB277716)
Interpolated concentrations of native IL-23R in human serum and plasma samples.
The concentrations of IL-23R were measured in duplicates, interpolated from the IL-23R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23R concentration was determined to be 174.9 pg/mL in serum, 128.8 pg/mL in plasma (EDTA), and 145.5 pg/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human IL-23R ELISA Kit (AB277716)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-23R concentration was determined to be 181.9 pg/mL with a range of 96.7 – 248.0 pg/mL.
Reactivity data
Product details
Human IL-23R ELISA kit (ab277716) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-23R protein in human serum, plasma, and cell culture supernatants. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-23R with 9.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-23 receptor, encoded by the IL23R gene, is a type I cytokine protein receptor that pairs together with IL-12Rβ1 as a complex in which IL-23 has a binding affinity for signaling innate and adaptive immunity. More specifically, IL-23R associates with Jak2 and Stat3 signaling molecules and is expressed by both T cells and NK cells. This kit is developed to recognize only IL-23R.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com