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AB267595

Human IL-24 ELISA Kit

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Human IL-24 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-24 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

MDA7, ST16, IL24, Interleukin-24, IL-24, Melanoma differentiation-associated gene 7 protein, Suppression of tumorigenicity 16 protein, MDA-7

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Sandwich ELISA - Human IL-24 ELISA Kit (AB267595)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-24 ELISA Kit (AB267595)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.41 ng/mL

Range

0.41 - 100 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-24 ELISA Kit is designed for the quantitative determination of IL-24 in cell culture supernatants, plasma and serum samples.

Δ Note: Human IL-24 concentration is low in normal serum/plasma, and may not be detectable in this assay. Collect plasma using EDTA or Citrate as an anticoagulant. Heparin is not recommended.

This assay employs an antibody specific for human IL-24 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-24 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-24 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-24 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Less than 0.2% cross-reactivity with mouse IL-24 is observed.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "69 - 75 %", "average": "= 73.92" }, { "sample": "Cell culture media", "range": "108 - 149 %", "average": "= 129.8" }, { "sample": "Plasma", "range": "67 - 85 %", "average": "= 73" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267595-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267595-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267595-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB267595-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-24 Standard (Lyophilized)", "number":"AB267595-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267595-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL-24 coated Microplate (12 x 8 wells)", "number":"AB267595-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267595-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human IL-24 Detection Antibody", "number":"AB267595-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267595-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis (PubMed : 25168428, PubMed : 27687232). Possesses antiviral functions and induces the type I interferon response during influenza infection (PubMed : 27687232). Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1 (PubMed : 11706020, PubMed : 30111632). In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1 (PubMed : 25168428). Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway-mediated stress signal (By similarity). In addition, acts as a quality control mechanism for the ubiquitin proteasome system by alerting the cell to proteasome dysfunction through activation of PKR/EIF2AK2 (By similarity).
See full target information IL24
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websiteProtocolBooklet
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