Human IL-24 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-24 in Human in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.41 - 100 ng/mL
- Sensitivity
- = 0.41 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis (PubMed:25168428, PubMed:27687232). Possesses antiviral functions and induces the type I interferon response during influenza infection (PubMed:27687232). Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1 (PubMed:11706020, PubMed:30111632). In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1 (PubMed:25168428). Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway-mediated stress signal (By similarity). In addition, acts as a quality control mechanism for the ubiquitin proteasome system by alerting the cell to proteasome dysfunction through activation of PKR/EIF2AK2 (By similarity).
Alternative names
MDA7, ST16, IL24, Interleukin-24, IL-24, Melanoma differentiation-associated gene 7 protein, Suppression of tumorigenicity 16 protein, MDA-7
What's included?
Recommended products
Human IL-24 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-24 in Human in Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.41 - 100 ng/mL
- Assay Platform
- Microplate
- Sensitivity
- = 0.41 ng/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 73.92 | Range 69 - 75 % |
Sample type Cell culture media | Average % = 129.8 | Range 108 - 149 % |
Sample type Plasma | Average % = 73 | Range 67 - 85 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human IL-24 ELISA Kit is designed for the quantitative determination of IL-24 in cell culture supernatants, plasma and serum samples.
Δ Note: Human IL-24 concentration is low in normal serum/plasma, and may not be detectable in this assay. Collect plasma using EDTA or Citrate as an anticoagulant. Heparin is not recommended.
This assay employs an antibody specific for human IL-24 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-24 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-24 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-24 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Less than 0.2% cross-reactivity with mouse IL-24 is observed.
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1 product image
Sandwich ELISA - Human IL-24 ELISA Kit (ab267595)
This standard curve is for demonstration only. A standard curve must be run with each assay.
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Product protocols
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