Human IL-25 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-25 with a sensitivity of 41.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- BMC pulmonary medicine 23:4222023Angiotensin-(1-7) suppresses airway inflammation and airway remodeling via inhibiting ATG5 in allergic asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianfeng Xu,Zhenyu Yu,Xueping LiuPubMed 37919667
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 94 - 6000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 41.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production (PubMed:15860795, PubMed:25821217). Promotes and augments T-helper type 2 responses locally or systemically (PubMed:25821217). Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction (By similarity). In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13 (PubMed:25821217). Induces also the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways (PubMed:15860795). Inhibits the differentiation of T-helper (Th17) cells via the production of IL4, IL5 and IL13 (PubMed:11754819).
Alternative names
IL17E, UNQ3120/PRO10272, IL25, Interleukin-25, IL-25, Interleukin-17E, IL-17E
What's included?
Recommended products
Human IL-25 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-25 with a sensitivity of 41.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Heparin Plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 94 - 6000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 41.8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 8.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 4 | mean - | SD - | C.V. 14.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 92 - 98 % |
Sample type EDTA Plasma | Average % = 96 | Range 95 - 97 % |
Sample type Cell culture media | Average % = 113 | Range 108 - 115 % |
Sample type Heparin Plasma | Average % = 87 | Range 86 - 87 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-25 ELISA kit (ab272200) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-25 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-25 with 41.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Interleukin-25, also known as IL-17E, is a cytokine that belongs to the IL-17 cytokine family and is encoded by the IL25 gene. IL-25 has a heterodimeric receptor composed of IL-17RA and IL-17RB, where both subunits are necessary for IL-25 functions and are expressed in the main Th2 cells. The main function of IL-25 is to regulate the adaptive immune responses by induction of several cytokines, such as; IL-4, IL-5, and IL-13. IL-25 supports the Th2 immune response and has been found to play a role in protecting against bowel infections by various intestinal parasites.
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2 product images
Sandwich ELISA - Human IL-25 ELISA Kit (ab272200)
Example of human IL-25 standard curve in Sample Diluent 50BP.
The IL-25 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-25 ELISA Kit (ab272200)
Interpolated concentrations of spiked human IL-25 in human serum, plasma and cell culture media samples.
The concentrations of IL-25 were measured in duplicates, interpolated from the IL-25 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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