JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB313976

Human IL-28 RA ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-28 RA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-28 RA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL28RA, LICR2, IFNLR1, Interferon lambda receptor 1, IFN-lambda receptor 1, IFN-lambda-R1, Cytokine receptor class-II member 12, Cytokine receptor family 2 member 12, Interleukin-28 receptor subunit alpha, Likely interleukin or cytokine receptor 2, CRF2-12, IL-28 receptor subunit alpha, IL-28R-alpha, IL-28RA

1 Images
Sandwich ELISA - Human IL-28 RA ELISA Kit (AB313976)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-28 RA ELISA Kit (AB313976)

Example of standard curve using ab313976. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.045 ng/mL

Range

0.045 - 30 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IL-28 RA ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL28 RA in serum (human IL-28 RA concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human IL-28 RA coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "89 - 133 %", "average": "= 114.9" }, { "sample": "Plasma", "range": "68 - 88 %", "average": "= 75.12" }, { "sample": "Cell culture media", "range": "135 - 145 %", "average": "= 141.3" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313976-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"900X HRP-Streptavidin", "number":"AB313976-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313976-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313976-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313976-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IL-28 RA Antibody-coated ELISA Plate", "number":"AB313976-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313976-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human IL-28 RA Detection Antibody", "number":"AB313976-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IL-28 RA Protein Standard", "number":"AB313976-CMP10", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL28RA also known as Interleukin-28 receptor alpha or IFNLR1 is a cytokine receptor subunit with a molecular mass of roughly 57 kDa. This protein is part of the class II cytokine receptor family and is expressed predominantly in epithelial cells but also found in liver tissues and certain immune cells. IL28RA forms a receptor complex with IL10RB facilitating signaling through binding with type III interferons such as IFN-λ1 IFN-λ2 and IFN-λ3.
Biological function summary

IL28RA plays an important role in mediating antiviral responses and regulating immune processes. As part of a heterodimeric receptor complex IL28RA along with IL10RB responds to type III interferons. These interactions trigger Jak-STAT signaling pathways which contribute to the transcriptional activation of interferon-stimulated genes (ISGs). The induction of these ISGs provides an important immune defense by inhibiting viral replication and modulating the immune response.

Pathways

IL28RA's function connects to the Jak-STAT signaling pathway and the innate immune response. In the Jak-STAT pathway IL28RA collaborates with IL10RB to activate STAT proteins and facilitate immune signaling. This pathway's activity is integral for maintaining antiviral defense mechanisms and modulating immune responses against pathogens. Through this pathway IL28RA links to other proteins such as STAT1 and STAT2 which play important roles in signal transduction.

Studies implicate IL28RA in conditions like chronic hepatitis C and autoimmune diseases. The role of IL28RA in immune modulation suggests its involvement in the pathogenesis of these conditions. In chronic hepatitis C IL28RA interacts with type III interferons influencing viral clearance and treatment responses. Furthermore in autoimmune disorders aberrant IL28RA signaling may contribute to dysregulated immune responses. Its interaction with IL10RB and subsequent signaling events could impact disease progression and therapeutic outcomes.
style
left-column

Product protocols

style
left-column

Target data

The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state. Determines the cell type specificity of the lambda interferon action. Shows a more restricted pattern of expression in the epithelial tissues thereby limiting responses to lambda interferons primarily to epithelial cells of the respiratory, gastrointestinal, and reproductive tracts. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium.
See full target information IFNLR1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Primary Antibodies

AB224395

Anti-IL28RA antibody

Western blot - Anti-IL28RA antibody (AB224395)

  • 0
  • 0
Proteins & Peptides

AB165762

Recombinant Human IL28RA protein

SDS-PAGE - Recombinant Human IL28RA protein (AB165762)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com