JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277438

Human IL-28B ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-28B ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-28B in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL28B, IL28C, ZCYTO22, IFNL3, Interferon lambda-3, IFN-lambda-3, Cytokine Zcyto22, Interleukin-28B, Interleukin-28C, IL-28B, IL-28C

1 Images
ELISA - Human IL-28B ELISA Kit (AB277438)
  • ELISA

Supplier Data

ELISA - Human IL-28B ELISA Kit (AB277438)

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.053 ng/mL

Range

0.051 - 12.5 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IL-28B ELISA Kit (ab277438) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28B in serum (human IL-28B concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Human IL-28B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human IL-28B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human IL-28B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human IL-28B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "General", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "112 - 145 %", "average": "= 132.9" }, { "sample": "Plasma", "range": "75 - 79 %", "average": "= 77.89" }, { "sample": "Cell culture media", "range": "82 - 96 %", "average": "= 87.64" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277438-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277438-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277438-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL-28B coated Microplate", "number":"AB277438-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277438-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277438-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human IL-28B Antibody", "number":"AB277438-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB277438-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB277438-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-28B standard protein (Lyophilized)", "number":"AB277438-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.
See full target information IFNL3
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB238269

Human IL-28A ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-28A ELISA Kit (AB238269)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB276441

Recombinant human IL-28B protein (His tag)

Functional Studies - Recombinant human IL-28B protein (His tag) (AB276441)

  • 0
  • 0
Primary Antibodies

AB181411

Anti-IL-28B antibody

Western blot - Anti-IL-28B antibody (AB181411)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com