AB275903

Human IL-2RB ELISA Kit (CD122)

Recombinant
SimpleStep
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Human IL-2RB ELISA Kit (CD122) is a single-wash 90-min Simplestep used to quantify Human IL-2RB (CD122) with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD122, IL15RB, IL2RB, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75, p75

4 Images

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

sELISA

Supplier Data

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2RB concentration was determined to be 234 pg/mL with a range of 101 – 392 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Interpolated concentrations of native IL-2RB in human serum and plasma samples.

The concentrations of IL-2RB were measured in duplicates, interpolated from the IL-2RB standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2RB concentration was determined to be 274 pg/mL in neat serum, 138 pg/mL in neat plasma (citrate), 155 pg/mL in neat plasma (EDTA) and 225 pg/mL in neat plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Interpolated concentrations of native IL-2RB in human PBMC cell culture supernatant samples treated with or without 1.5% PHA-M for 36 hours.

The concentrations of IL-2RB were measured in duplicates, interpolated from the IL-2RB standard curves and corrected for sample dilution. Undiluted samples are as follows : PHA treated PBMC supernatant 100% and untreated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-2RB concentration was determined to be 186 pg/mL in neat PBMC supernatant PHA-M treated and undetectable in untreated PBMC supernatant.

sELISA

Supplier Data

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Example of human IL-2RB standard curve in Sample Diluent NS.

The IL-2RB standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, EDTA Plasma, Heparin Plasma, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8 pg/mL

Range

43.75 - 2800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL-2RB ELISA kit (ab275903) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-2RB protein in human serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-2RB with 8 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-2RB (Interleukin-2 receptor subunit beta), also known as CD122, is a receptor for interleukin-2. IL-2 receptor exists in 3 different forms: a high affinity non-covalent dimer of an alpha and a beta subunit, an intermediate affinity monomer of beta subunit, and a low affinity monomer of alpha subunit. The high and intermediate affinity forms can also associate with a gamma subunit. The beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]

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Recovery

[ { "sample": "Serum", "range": "80 - 86 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "87 - 90 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "95 - 104 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "84 - 97 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "79 - 84 %", "average": "= 81" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IL2 Receptor beta also known as IL-2R beta IL2R beta or CD122/IL2RB protein is a component of the interleukin-2 receptor complex. It is part of the receptor system for the cytokine IL-2. This receptor subunit has a molecular mass of approximately 75 kDa and gets alternatively referred to as IL2R beta or p75. It is expressed on the surface of activated T cells natural killer (NK) cells and in some cases myeloid lineage cells. This expression pattern correlates IL2R beta's role in essential immune functions and signaling processes.

Biological function summary

IL2R beta plays a critical role in cytokine binding and signal transduction for the interleukin-2 receptor which is a heterodimeric complex. This complex consists of the IL-2 receptor alpha (CD25) IL-2 receptor beta and a common gamma chain (γc CD132). The presence of IL-2R beta allows the receptor to mediate biological processes like cell proliferation differentiation and survival particularly within the immune system. The extent to how IL2R beta integrates into these processes makes it an important player in immune regulation and homeostasis.

Pathways

IL2R beta serves essential functions in the JAK-STAT and PI3K-AKT pathways. The binding of IL-2 to its receptor initiates a cascade of intracellular events which involve Janus kinases (JAKs) and signal transducer and activator of transcription (STATs) leading to transcriptional activation of various genes. Along with these pathways IL2R beta also interacts collaboratively with the gamma chain (γc) in promoting Downstream signaling events which enhance cell proliferation and survival.

IL2R beta's function has implications in autoimmune diseases and certain types of leukemia. Alterations or dysregulation in IL-2R signaling pathways often play a role in autoimmune conditions due to its importance in maintaining immune tolerance. Moreover given IL-2 receptor's role in T-cell and NK-cell activity aberrations may contribute to uncontrolled cellular proliferation seen in leukemia. Understanding its interplay with the common gamma chain (CD132) can provide insights into therapeutic interventions targeting these disorders.

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Product protocols

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Target data

Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed : 15123770, PubMed : 31040185).

See full target information IL2RB

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-2RB ELISA Kit (CD122)

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Sandwich ELISA - Human IL-2RB ELISA Kit (CD122) (AB275903)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise