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AB215088

Human IL-3 ELISA Kit

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Human IL-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-3 with a sensitivity of 6.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-3, IL-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, MCGF, IL3

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ELISA - Human IL-3 ELISA Kit (AB215088)
  • ELISA

Supplier Data

ELISA - Human IL-3 ELISA Kit (AB215088)
Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)

Interpolated concentrations of spike IL-3 in RPMI media and human urine.

The concentrations of IL-3 were measured in duplicate and interpolated from the IL-3 standard curve and corrected for sample dilution. Undiluted samples are as follows : RPMI+10% FBS 50%, urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)
  • sELISA

Unknown

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)

Human IL-3 standard curve comparison data.

Standard curve comparison between human IL-3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)

Interpolated concentrations of native IL-3 in human PBMC cell culture supernatant samples.

Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin. Cells were cultured for 2 days at 37°C in the presence or absence of PHAM. The concentrations of IL-3 were measured in duplicates, interpolated from the IL-3 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated supernatant 100% and unstimulated supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean (IL-3 concentration was determined to be 662 pg/mL in 100% stimulated supernatant, and 9.5 pg/mL in 100% unstimulated supernatant.

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)

Example of human IL-3 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-3 ELISA Kit (AB215088)

Interpolated concentrations of spike IL-3 in human serum and plasmas.

The concentrations of IL-3 were measured in duplicate and interpolated from the IL-3 standard curve and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (Heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Urine, Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.7 pg/mL

Range

15 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-3 ELISA Kit ab215088 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-3 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, plasma, serum, urine. This SimpleStep sensitivity is 6.7 pg/mL.

How the assay works

Human IL-3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-3 ELISA Kit ab215088 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-3 antibody [EPR19870-164] - BSA and Azide free (Capture) ab244900
- Anti-IL-3 antibody [EPR19870-40] - BSA and Azide free (Detector) ab244900

Interleukin 3 (IL-3) is a secreted 133 amino acids long, glycosylated interleukin coded by the IL-3 gene. Previous names reported for IL-3 are Hematopoietic growth factor, Mast cell growth factor, multipotenial colony-stimulating factor, and P-cell-stimulating factor. IL-3 has been found to stimulate a variety of cell activities such as; proliferation, differentiation, survival of pluripotent hematopoietic stem cells, and may be associated with neurologic disorders. IL-3 is expressed by activated T-cells, mast cells, and natural killer cells. IL-3 performs its biological action by binding to a transmembrane receptor complex consisting of IL-3RA, also known as CD123, and a signal inducing beta subunit, which also acts as a receptor for colony stimulating factor 2 and Interleukin 5.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Serum", "range": "84 - 91 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "87 - 89 %", "average": "= 89" }, { "sample": "Urine", "range": "88 - 92 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "81 - 92 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "84 - 87 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "94 - 103 %", "average": "= 99" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab215088-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB215088-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB215088-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-3 Lyophilized Recombinant Protein", "number":"AB215088-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB215088-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB215088-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-3 Capture Antibody", "number":"AB215088-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-3 Detector Antibody", "number":"AB215088-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB215088-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB215088-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB215088-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 2556442). Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated (PubMed : 10779277, PubMed : 32889153). In addition, plays an important role in neural cell proliferation and survival (PubMed : 23226269). Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation (PubMed : 12816992). Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB (PubMed : 29374162). Receptor stimulation results in the rapid activation of JAK2 kinase activity leading to STAT5-mediated transcriptional program (By similarity). Alternatively, contributes to cell survival under oxidative stress in non-hematopoietic systems by activating pathways mediated by PI3K/AKT and ERK (PubMed : 27862234).
See full target information IL3
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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