JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235637

Human IL-31 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-31 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-31 with a sensitivity of 1.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-31, IL-31, IL31

3 Images
Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Interpolated concentrations of spike IL-31 in human serum, plasma (EDTA), and cell culture media (RPMI containing 10% FBS) samples.

The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (EDTA) 25%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Example of human IL-31 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-31 ELISA Kit (AB235637)

Example of human IL-31 standard curve in Sample Diluent 50BS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.5 pg/mL

Range

117.2 - 7500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IL-31 ELISA Kit (ab235637) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-31 with 1.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "98 - 102 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "92 - 98 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 105" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab235637-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB235637-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB235637-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB235637-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB235637-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-31 Capture Antibody", "number":"AB235637-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB235637-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB235637-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB235637-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-31 Lyophilized Recombinant Protein", "number":"AB235637-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB235637-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-31 Detector Antibody", "number":"AB235637-CMP04", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR (PubMed : 15184896). May function in skin immunity (PubMed : 15184896). Enhances myeloid progenitor cell survival in vitro (By similarity). Induces RETNLA and serum amyloid A protein expression in macrophages (By similarity).
See full target information IL31
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB215089

Human IL-4 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-4 ELISA Kit (AB215089)

  • 5
  • 1
Assay Kits

AB288591

Human IL-13 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-13 ELISA Kit (AB288591)

  • 0
  • 0
Assay Kits

AB119542

Human IL-21 ELISA Kit

Sandwich ELISA - Human IL-21 ELISA Kit (AB119542)

  • 0
  • 0
Assay Kits

AB223865

Human IL-33 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-33 ELISA Kit (AB223865)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com