Human IL-32 alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-32 alpha in Human in Plasma, Cell culture media, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.1 - 1000 pg/mL
- Sensitivity
- = 4.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
Alternative names
NK4, TAIF, IL32, Interleukin-32, IL-32, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor
What's included?
Recommended products
Human IL-32 alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-32 alpha in Human in Plasma, Cell culture media, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture media, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.1 - 1000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 4.1 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 10 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 113.4 | Range 102 - 123 % |
Sample type Plasma | Average % = 112.9 | Range 101 - 119 % |
Sample type Cell culture media | Average % = 83.07 | Range 79 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human IL-32 alpha ELISA Kit is designed for the quantitative determination of IL-32 alpha in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human IL-32 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-32 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-32 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-32 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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1 product image
Sandwich ELISA - Human IL-32 alpha ELISA Kit (ab267616)
These standard curves are for demonstration only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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