JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB223865

Human IL-33 ELISA Kit

Be the first to review this product! Submit a review

|

(7 Publications)

Human IL-33 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-33 with a sensitivity of 4.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

C9orf26, IL1F11, NFHEV, IL33, Interleukin-33, IL-33, Interleukin-1 family member 11, Nuclear factor from high endothelial venules, IL-1F11, NF-HEV

7 Images
ELISA - Human IL-33 ELISA Kit (AB223865)
  • ELISA

Supplier Data

ELISA - Human IL-33 ELISA Kit (AB223865)
Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)

Example of human IL33 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)

Interpolated concentrations of spike IL33 in human serum, plasma and cell culture media (RPMI + 10% FBS) samples.

The concentrations of IL33 were measured in duplicates, interpolated from the IL33 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum neat, plasma (heparin) neat, plasma (EDTA) neat and RPMI + 10% FBS neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)

Interpolated concentrations of native IL33 in human PMA/LPS stimulated THP-1 cell extract based on a 1,000 μg/mL extract load.

The concentrations of IL33 were measured in duplicate and interpolated from the IL33 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL33 concentration was determined to be 351.5 pg/mL in PMA/LPS stimulated THP-1 cell extract and undetectable in unstimulated THP-1 cell extract (not shown).

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)

Comparison of IL33 in unstimulated and PMA/LPS stimulated THP-1 cell supernatants.

THP-1 cells were cultured for 24 hours in the presence or absence of 50 ng/mL PMA and 10 μg/mL LPS. The concentrations of IL33 were measured in neat supernatant samples in quadruplicates and interpolated from the IL33 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean IL33 concentration was determined to be 15.95 pg/mL in neat PMA/LPS stimulated THP-1 cell culture supernatant and undetectable in neat unstimulated THP-1 cell culture supernatant and RPMI media (not shown).

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)

Example of human IL33 standard curve in Sample Diluent 50BP.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-33 ELISA Kit (AB223865)

Example of human IL33 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 4.7 pg/mL

Range

11.72 - 750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IL-33 ELISA Kit ab223865 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-33 in cell culture extracts, cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 4.7 pg/mL.

How the assay works

Human IL-33 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-33 ELISA Kit ab223865 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-33 antibody [EPR20417-94] - BSA and Azide free (Capture) ab244933
- Anti-IL-33 antibody [EPR20417-53] - BSA and Azide free (Detector) ab244933

IL-33 (Interleukin-33, also known as IL-1F11 or NF-HEV) is a proinflammatory cytokine member of the IL-1 family. It is widely expressed and functions by binding and signaling through the IL-1RL1/ST2 receptor. IL-33 is expressed as a pro-protein; the antibodies in this kit were generated using the mature form of IL-33 (aa 109-270).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "83 - 95 %", "average": "= 87" }, { "sample": "Cell culture extracts", "range": "92 - 100 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "96 - 108 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "100 - 112 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "97 - 110 %", "average": "= 102" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab223865-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB223865-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB223865-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB223865-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB223865-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB223865-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB223865-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-33 Lyophilized Recombinant Protein", "number":"AB223865-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BP", "number":"AB223865-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-33 Capture Antibody", "number":"AB223865-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB223865-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB223865-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB223865-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-33 Detector Antibody", "number":"AB223865-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed : 16286016, PubMed : 19841166). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (PubMed : 17853410, PubMed : 18836528). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (PubMed : 17853410, PubMed : 18836528). Acts as an enhancer of polarization of alternatively activated macrophages (PubMed : 19841166). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (PubMed : 17853410, PubMed : 18836528). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity).. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed : 21734074). This form is rapidely lost upon angiogenic or pro-inflammatory activation (PubMed : 18787100).
See full target information IL33
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 136: PubMed37051862

2023

Inflammatory macrophage to hepatocyte signals can be prevented by extracellular vesicle reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Ghosh,Kyo Sasaki,Isabel Aranzazu Pulido Ruiz,Kayla E King,Steven A Weinman,Ann L Wozniak

Inflammation 46:1430-1444 PubMed37154978

2023

TLR7 Agonists Modulate the Activation of Human Conjunctival Epithelial Cells Induced by IL-1β via the ERK1/2 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wang,Shixu Li,Kaihong Cai,Yu Xiao,Lin Ye

Bioengineered 12:11738-11755 PubMed34895039

2021

The long noncoding RNA noncoding RNA activated by DNA damage (NORAD)-microRNA-496-Interleukin-33 axis affects carcinoma-associated fibroblasts-mediated gastric cancer development.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoqun Huang,Jiuyang Liu,Liang He,Fubing Wang,Bin Xiong,Yan Li,Xiaojun Yang

Journal of musculoskeletal & neuronal interactions 20:437-443 PubMed32877981

2020

Bone changes and curative effect of infliximab in patients with ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Linying Li,Bin Chen,Haiyan Zhao,Gengshen Wang

Cell cycle (Georgetown, Tex.) 19:1132-1142 PubMed32298206

2020

Epithelial cells expressed IL-33 to promote degranulation of mast cells through inhibition on ST2/PI3K/mTOR-mediated autophagy in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Bin Nian,Min Zeng,Jing Zheng,Lian-Ya Zeng,Zhi Fu,Qiu-Ju Huang,Xin Wei

Open medicine (Warsaw, Poland) 14:625-628 PubMed31535033

2019

Metformin Can Alleviate the Symptom of Patient with Diabetic Nephropathy Through Reducing the Serum Level of Hcy and IL-33.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhang,Jiansheng Niu,Xiumei Zhang,Wanxia He

Arthritis & rheumatology (Hoboken, N.J.) 72:166-178 PubMed31339007

2019

Activated M2 Macrophages Contribute to the Pathogenesis of IgG4-Related Disease via Toll-like Receptor 7/Interleukin-33 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Noriko Ishiguro,Masafumi Moriyama,Katsuhiro Furusho,Sachiko Furukawa,Takuma Shibata,Yusuke Murakami,Akira Chinju,A S M Rafiul Haque,Yuka Gion,Miho Ohta,Takashi Maehara,Akihiko Tanaka,Masaki Yamauchi,Mizuki Sakamoto,Keita Mochizuki,Yuko Ono,Jun-Nosuke Hayashida,Yasuharu Sato,Tamotsu Kiyoshima,Hidetaka Yamamoto,Kensuke Miyake,Seiji Nakamura
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB214025

Human IL-1 beta ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

  • 4
  • 1
Assay Kits

AB272200

Human IL-25 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-25 ELISA Kit (AB272200)

  • 0
  • 0
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com