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AB267633

Human IL-36 gamma ELISA (IL-1F9)

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Human IL-36 gamma ELISA (IL-1F9) is a Sandwich (quantitative) ELISA for the measurement of Human IL-36 gamma ELISA (IL-1F9) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1E, IL1F9, IL1H1, IL1RP2, UNQ2456/PRO5737, IL36G, Interleukin-36 gamma, IL-1-related protein 2, Interleukin-1 epsilon, Interleukin-1 family member 9, Interleukin-1 homolog 1, IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1

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Sandwich ELISA - Human IL-36 gamma ELISA (IL-1F9) (AB267633)
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Supplier Data

Sandwich ELISA - Human IL-36 gamma ELISA (IL-1F9) (AB267633)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 13 pg/mL

Range

12.29 - 3000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Human IL-36 gamma ELISA Kit is designed for the quantitative determination of IL-36 gamma in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human IL-36 gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-36 gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-36 gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-36 gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human IL-1F1 (IL-1 alpha), IL-1F2 (IL-1 beta), IL-1F3 (IL-1ra), IL-1F4 (IL-18), IL-1F5 (IL-36ra), IL-1F6 (IL-36 alpha), IL-1F7 (IL-37), IL-1F8 (IL-36 beta), IL-1F10 (IL-38), IL-1F11 (IL-33).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "110 - 147 %", "average": "= 120.3" }, { "sample": "Plasma", "range": "100 - 114 %", "average": "= 108.4" }, { "sample": "Cell culture media", "range": "99 - 116 %", "average": "= 107.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267633-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267633-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267633-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267633-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267633-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human IL-36 gamma Detection Antibody", "number":"AB267633-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL-36 gamma coated Microplate (12 x 8 wells)", "number":"AB267633-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-36 gamma Standard (Lyophilized)", "number":"AB267633-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB267633-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL36 gamma also known as IL-1F9 or IL-36 is a member of the interleukin-1 cytokine family. The protein weighs approximately 22 kDa. It is expressed highly in epithelial tissues such as keratinocytes in the skin bronchial tissues and intestinal lining. It acts mainly on nearby cells to influence immune responses through cytokine signaling.
Biological function summary

IL36 gamma plays a role in the immune and inflammatory responses. It operates as a cytokine that signals through the IL-1 receptor family leading to the activation of NF-kB and MAPK pathways. This member of the IL-1 family does not form a complex by itself but interacts with IL-36R to exert its functions. The protein influences processes such as cell proliferation differentiation and the regulation of immune responses to external pathogens or injuries.

Pathways

IL36 gamma is involved in inflammatory and immune signaling pathways. Within the immune response pathway it stimulates the production of other cytokines and pro-inflammatory molecules. IL36 gamma is associated with the IL-1 signaling pathway alongside other cytokines such as IL-1 and IL-18 which all contribute to immune modulation and inflammatory responses.

IL36 gamma has connections to psoriasis and inflammatory bowel disease. In psoriasis increased levels of IL36 gamma correlate with heightened skin inflammation and proliferation of keratinocytes. In inflammatory bowel disease IL36 gamma contributes to the inflammatory state of the gut. The activities of IL36 gamma often link with other cytokines such as TNF-alpha which collectively drive the inflammatory processes observed in these conditions.
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Product protocols

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Target data

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous pro-inflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation : activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
See full target information IL36G
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websiteProtocolBooklet
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