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Human IL-36R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-36R in Human in Serum, Plasma, Cell culture supernatant samples.

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Sandwich ELISA - Human IL-36R ELISA Kit (AB309451), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
24 - 6000 pg/mL
Sensitivity
= 24 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways (By similarity). The IL-36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. Seems to be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
250X HRP-Streptavidin
1 x 200 µL
5X Assay Diluent (Item E2)
1 x 15 mL
96-Well Plate Sealing Film
1 x 1 Unit
Biotinylated Human IL-1 R6 Detection Antibody
2 x 1 Vial
Human IL-1 R6 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human IL-1 R6 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human IL-36R ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-36R in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
24 - 6000 pg/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 24 pg/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 77.67
Range
68 - 96 %
Sample type
Plasma
Average %
= 76.68
Range
68 - 80 %
Sample type
Cell culture supernatant
Average %
= 78.91
Range
72 - 86 %
Sample type
Cell culture media
Average %
= 78.91
Range
72 - 86 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human IL-36R ELISA Kits an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-36R in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IL-36R coated on a 96-well plate.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human IL-36R ELISA Kit (ab309451), expandable thumbnail

    Sandwich ELISA - Human IL-36R ELISA Kit (ab309451)

    Example of standard curve using ab309451. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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