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AB309451

Human IL-36R ELISA Kit

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Human IL-36R ELISA Kit is a Sandwich ELISA for the measurement of Human IL-36R in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1RRP2, IL1RL2, Interleukin-1 receptor-like 2, IL-36 receptor, Interleukin-1 receptor-related protein 2, IL-36R, IL-1Rrp2, IL1R-rp2

1 Images
Sandwich ELISA - Human IL-36R ELISA Kit (AB309451)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-36R ELISA Kit (AB309451)

Example of standard curve using ab309451. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 24 pg/mL

Range

24 - 6000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human IL-36R ELISA Kits an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-36R in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human IL-36R coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "68 - 96 %", "average": "= 77.67" }, { "sample": "Plasma", "range": "68 - 80 %", "average": "= 76.68" }, { "sample": "Cell culture supernatant", "range": "72 - 86 %", "average": "= 78.91" }, { "sample": "Cell culture media", "range": "72 - 86 %", "average": "= 78.91" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309451-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309451-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309451-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309451-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin", "number":"AB309451-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IL-1 R6 Protein Standard", "number":"AB309451-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309451-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human IL-1 R6 Detection Antibody", "number":"AB309451-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IL-1 R6 Antibody-coated ELISA Plate", "number":"AB309451-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB309451-CMP07", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Receptor for interleukin-36 (IL36A, IL36B and IL36G). After binding to interleukin-36 associates with the coreceptor IL1RAP to form the interleukin-36 receptor complex which mediates interleukin-36-dependent activation of NF-kappa-B, MAPK and other pathways (By similarity). The IL-36 signaling system is thought to be present in epithelial barriers and to take part in local inflammatory response; it is similar to the IL-1 system. Seems to be involved in skin inflammatory response by induction of the IL-23/IL-17/IL-22 pathway.
See full target information IL1RL2
websiteProtocolBooklet
en

Product promise

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