AB213798

Human IL-37 ELISA Kit

Name: Human IL-37 ELISA Kit (ab213798) | Abcam
Brand: Abcam Limited
SKU: AB213798
Price: 780 USD
Availability: InStock

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(5 Publications)

Human IL-37 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-37 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

FIL1Z, IL1F7, IL1H4, IL1RP1, IL37, Interleukin-37, IL-37, FIL1 zeta, IL-1X, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, IL-1F7, IL-1H, IL-1H4, IL-1 zeta, IL-1RP1

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Sandwich ELISA - Human IL-37 ELISA Kit (AB213798)

sELISA

Supplier Data

Sandwich ELISA - Human IL-37 ELISA Kit (AB213798)

Human IL-37 ELISA Kit (ab213798) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

15.6 - 1000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

The Human IL-37 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213798) is designed for the quantitative measurement of Human IL-37 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for IL-37 has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: V46-D218) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-37 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-37 amount of sample captured in plate.

Interleukin 37 also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family, and it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "216 pg/mL", "standardDeviation": "12.3", "coefficientOfVariability": "5.7" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "365 pg/mL", "standardDeviation": "15.33", "coefficientOfVariability": "4.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "610 pg/mL", "standardDeviation": "39.04", "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "178 pg/mL", "standardDeviation": "7.3", "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "304 pg/mL", "standardDeviation": "11.86", "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "569 pg/mL", "standardDeviation": "30.2", "coefficientOfVariability": "5.3" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-37 also known as IL-1F7 is a member of the interleukin 1 cytokine family. This protein has a molecular mass of approximately 25 kDa. IL-37 is mainly expressed in tissues like the kidney thymus testis and uterus with lower levels in the bone marrow and placenta. Researchers identify this protein in various cells such as monocytes which are key players in the immune system.

Biological function summary

IL-37 functions within the immune response by acting as a critical anti-inflammatory cytokine. It does not form a larger complex for its activity but instead interacts directly with various immune cells. IL-37 is capable of reducing pro-inflammatory cytokine production and dampening the immune response when necessary thereby providing a mechanism to prevent excessive inflammation and tissue damage.

Pathways

IL-37 plays a role in pathways like the inflammatory response and immune regulation. Within these systems it interacts with proteins such as IL-18 and IL-18BP (binding protein) which helps modulate its activity. IL-37 can also influence the NF-kB pathway an essential pathway in regulating the immune response by indirectly reducing inflammation.

IL-37 is associated with conditions such as rheumatoid arthritis and inflammatory bowel disease. These disorders involve chronic inflammation where IL-37 might serve a protective role by reducing excessive inflammatory signals. In these contexts IL-37 interacts with other inflammatory cytokines like IL-6 and TNF-α which are known to be elevated in such diseases. By potentially mitigating these signals IL-37 helps in managing the inflammatory aspects of these disorders.

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Product protocols

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Target data

Immune regulatory cytokine that acts as a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. Signaling can occur via two mechanisms, intracellularly through nuclear translocation with SMAD3 and extracellularly after secretion and binding to its receptor composed of IL18R1 and IL18RAP. Suppresses, or reduces, pro-inflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.

See full target information IL37

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:7116 PubMed39164237

2024

Toripalimab plus chemotherapy and radiotherapy for treatment-naive advanced esophageal squamous cell carcinoma: a single-arm phase 2 trial.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Baisen Li,Gang Wan,Yi Wang,Jie Zhu,Long Liang,Xuefeng Leng,Wenwu He,Lin Peng,Yongtao Han,Shuya He,Dongsheng Wang,Yehan Zhou,Liang Yi,Wencheng Zhang,Qingsong Pang,Wei Zhang,Tao Li,Jinyi Lang,Yang Liu,Bangrong Cao,Qifeng Wang

International journal of immunopathology and pharmacology 37:3946320231209821 PubMed37953627

2023

Correlation analysis between cytokines' profile, autoimmune antibodies and the duration of type 1 diabetes: A case control study in a specialized children's centre in Riyadh.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Al-Dubayee,Amir Babiker,Abdulaziz Alkewaibeen,Abdulrahman Alkhalifah,Tammam Alanazi,Maysa Nogoud,Anas Alotaibi,Faisal Alotaibi,Firas Almetairi,Mohammed A Alrowaily,Emad Masuadi,Amre Nasr

Kidney diseases (Basel, Switzerland) 9:104-117 PubMed37065609

2023

IL-37 Ameliorates Renal Fibrosis by Restoring CPT1A-Mediated Fatty Acid Oxidation in Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xiong,Ting He,Chi Liu,Shaozong Qin,Tangli Xiao,Wang Xin,Yaqin Wang,Li Ran,Bo Zhang,Jinghong Zhao

Animal models and experimental medicine 4:268-277 PubMed34557653

2021

New rat to mouse xenograft transplantation of endometrium as a model of human endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Amir Abdolmaleki,Cyrus Jalili,Kamran Mansouri,Mitra Bakhtiari

Journal of the American Society of Nephrology : JASN 31:54-65 PubMed31604808

2019

Additive Therapeutic Effects of Mesenchymal Stem Cells and IL-37 for Systemic Lupus Erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyong Xu,Jieting Chen,Wenlei Li,Wei Lian,Jieyong Huang,Baoyu Lai,Lingyun Li,Zhong Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human IL-37 ELISA Kit

Sandwich ELISA - Human IL-37 ELISA Kit (AB213798)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise