Human IL-37 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-37 with a sensitivity of 8.016 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Publications
Filter by
- American journal of translational research 14:9018-90242022The diagnostic value of combined detection of microRNA-155, TNF-α and IL-37 for active pulmonary tuberculosis in the elderly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLongmei Yu,Haiyang Fu,Haiyun ZhangPubMed 36628207
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.875 - 3000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 8.016 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Immune regulatory cytokine that acts as a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. Signaling can occur via two mechanisms, intracellularly through nuclear translocation with SMAD3 and extracellularly after secretion and binding to its receptor composed of IL18R1 and IL18RAP. Suppresses, or reduces, pro-inflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
Alternative names
FIL1Z, IL1F7, IL1H4, IL1RP1, IL37, Interleukin-37, IL-37, FIL1 zeta, IL-1X, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, IL-1F7, IL-1H, IL-1H4, IL-1 zeta, IL-1RP1
What's included?
Recommended products
Human IL-37 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-37 with a sensitivity of 8.016 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 46.875 - 3000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8.016 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 7.2 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture media | Average % = 98 | Range 90 - 107 % |
Sample type Citrate plasma | Average % = 94 | Range 81 - 116 % |
Sample type EDTA Plasma | Average % = 96 | Range 88 - 107 % |
Sample type Heparin Plasma | Average % = 98 | Range 90 - 105 % |
Sample type Serum | Average % = 99 | Range 92 - 109 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL-37 also known as IL-1F7 is a member of the interleukin 1 cytokine family. This protein has a molecular mass of approximately 25 kDa. IL-37 is mainly expressed in tissues like the kidney thymus testis and uterus with lower levels in the bone marrow and placenta. Researchers identify this protein in various cells such as monocytes which are key players in the immune system.
- Biological function summary
IL-37 functions within the immune response by acting as a critical anti-inflammatory cytokine. It does not form a larger complex for its activity but instead interacts directly with various immune cells. IL-37 is capable of reducing pro-inflammatory cytokine production and dampening the immune response when necessary thereby providing a mechanism to prevent excessive inflammation and tissue damage.
- Pathways
IL-37 plays a role in pathways like the inflammatory response and immune regulation. Within these systems it interacts with proteins such as IL-18 and IL-18BP (binding protein) which helps modulate its activity. IL-37 can also influence the NF-kB pathway an essential pathway in regulating the immune response by indirectly reducing inflammation.
- Associated diseases and disorders
IL-37 is associated with conditions such as rheumatoid arthritis and inflammatory bowel disease. These disorders involve chronic inflammation where IL-37 might serve a protective role by reducing excessive inflammatory signals. In these contexts IL-37 interacts with other inflammatory cytokines like IL-6 and TNF-α which are known to be elevated in such diseases. By potentially mitigating these signals IL-37 helps in managing the inflammatory aspects of these disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sandwich ELISA - Human IL-37 ELISA Kit (ab300313)
Example of Human IL-37 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com