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AB300313

Human IL-37 ELISA Kit

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(1 Publication)

Human IL-37 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-37 with a sensitivity of 8.016 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FIL1Z, IL1F7, IL1H4, IL1RP1, IL37, Interleukin-37, IL-37, FIL1 zeta, IL-1X, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, IL-1F7, IL-1H, IL-1H4, IL-1 zeta, IL-1RP1

3 Images
Sandwich ELISA - Human IL-37 ELISA Kit (AB300313)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-37 ELISA Kit (AB300313)

Recombinant IL-37 was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-37 ELISA Kit (AB300313)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-37 ELISA Kit (AB300313)

Recombinant IL-37 was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IL-37 ELISA Kit (AB300313)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-37 ELISA Kit (AB300313)

Example of Human IL-37 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.016 pg/mL

Range

46.875 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "90 - 107 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "81 - 116 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "88 - 107 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "90 - 105 %", "average": "= 98" }, { "sample": "Serum", "range": "92 - 109 %", "average": "= 99" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300313-96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300313-CMP19", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300313-CMP18", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300313-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300313-CMP17", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300313-CMP16", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-37 Capture Antibody", "number":"AB300313-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-37 Lyophilized Recombinant Protein", "number":"AB300313-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB300313-CMP14", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300313-CMP20", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-37 Detector Antibody", "number":"AB300313-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-37 also known as IL-1F7 is a member of the interleukin 1 cytokine family. This protein has a molecular mass of approximately 25 kDa. IL-37 is mainly expressed in tissues like the kidney thymus testis and uterus with lower levels in the bone marrow and placenta. Researchers identify this protein in various cells such as monocytes which are key players in the immune system.
Biological function summary

IL-37 functions within the immune response by acting as a critical anti-inflammatory cytokine. It does not form a larger complex for its activity but instead interacts directly with various immune cells. IL-37 is capable of reducing pro-inflammatory cytokine production and dampening the immune response when necessary thereby providing a mechanism to prevent excessive inflammation and tissue damage.

Pathways

IL-37 plays a role in pathways like the inflammatory response and immune regulation. Within these systems it interacts with proteins such as IL-18 and IL-18BP (binding protein) which helps modulate its activity. IL-37 can also influence the NF-kB pathway an essential pathway in regulating the immune response by indirectly reducing inflammation.

IL-37 is associated with conditions such as rheumatoid arthritis and inflammatory bowel disease. These disorders involve chronic inflammation where IL-37 might serve a protective role by reducing excessive inflammatory signals. In these contexts IL-37 interacts with other inflammatory cytokines like IL-6 and TNF-α which are known to be elevated in such diseases. By potentially mitigating these signals IL-37 helps in managing the inflammatory aspects of these disorders.
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Product protocols

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Target data

Immune regulatory cytokine that acts as a suppressor of innate inflammatory and immune responses involved in curbing excessive inflammation. Signaling can occur via two mechanisms, intracellularly through nuclear translocation with SMAD3 and extracellularly after secretion and binding to its receptor composed of IL18R1 and IL18RAP. Suppresses, or reduces, pro-inflammatory cytokine production, including IL1A and IL6, as well as CCL12, CSF1, CSF2, CXCL13, IL1B, IL23A and IL1RN, but spares anti-inflammatory cytokines. Inhibits dendritic cell activation.
See full target information IL37
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 14:9018-9024 PubMed36628207

2022

The diagnostic value of combined detection of microRNA-155, TNF-α and IL-37 for active pulmonary tuberculosis in the elderly.

Applications

Unspecified application

Species

Unspecified reactive species

Longmei Yu,Haiyang Fu,Haiyun Zhang
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Product promise

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