Human IL-4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-4 in Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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- Experimental and therapeutic medicine 26:3592023CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Wen Gao,Han-Lin Hu,Ming-Hua Xie,Cai-Xia Tang,Jun Ou,Zheng-Hao LuPubMed 37324510
- American journal of translational research 15:1360-13662023Therapeutic effect of modified Xiaoqinglong Decoction on cough-variant asthma and immunological functioning in children.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Li,Ying HanPubMed 36915768
- American journal of translational research 14:6874-68882022IFN-γ promotes the development of systemic lupus erythematosus through the IFNGR1/2-PSTAT1-TBX21 signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Bailing TianPubMed 36398225
- Journal of clinical medicine 11:2022Serum IL-13 Predicts Response to Golimumab in Bio-Naïve Ulcerative Colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaohiko Kinoshita,Kazuki Kakimoto,Hikaru Shimizu,Koji Nishida,Keijiro Numa,Yuka Kawasaki,Hideki Tawa,Kei Nakazawa,Ryoji Koshiba,Yuki Hirata,Naokuni Sakiyama,Eiko Koubayashi,Toshihisa Takeuchi,Takako Miyazaki,Kazuhide Higuchi,Shiro Nakamura,Hiroki NishikawaPubMed 36078882
- Advances in clinical and experimental medicine : official organ Wroclaw Medical University 31:1011-10222022MiR-218 promotes oxidative stress and inflammatory response by inhibiting SPRED2-mediated autophagy in HG-induced HK-2 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanfang Fu,Xinxin Huang,Juyun Zhang,Zhu Lin,Guijun QinPubMed 35506187
- Archives of gynecology and obstetrics 307:169-1772022miR-424-5p combined with miR-17-5p has high diagnostic efficacy for endometriosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunli Lin,Saili Zeng,Miaojie LiPubMed 35366691
- Cytokine 153:1558682022Clinical monitored in subjects metabolically healthy and unhealthy before and during a SARS-CoV-2 infection- A cross-sectional study in Mexican population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Treviño,Steffany Cortezano-Esteban,Hugo Hernández-Fragoso,Alfonso Díaz,Rubén Vázquez-Roque,Victor Enrique Sarmiento-Ortega,Diana Moroni-González,Rosana Pelayo,Eduardo BrambilaPubMed 35358903
- Pathobiology : journal of immunopathology, molecular and cellular biology 89:135-1452021Emodin Promotes Autophagy and Prevents Apoptosis in Sepsis-Associated Encephalopathy through Activating BDNF/TrkB Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Li Gao,Zhi-Hao Wang,Yu-Hang Mu,Zuo-Long Liu,Li PangPubMed 34872094
- Clinical and translational medicine 11:e4642021c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Zhang,Xiaohui Wang,Yunfei Li,Jing Han,Xianzheng Gao,Shenglei Li,Feng WangPubMed 34586738
- Experimental and therapeutic medicine 22:7442021miR-489-3p inhibits TLR4/NF-κB signaling to prevent inflammation in psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujian Ye,Ping Wang,Fangmei ZhouPubMed 34055060
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.08 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:1993171, PubMed:3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed:2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed:10219247, PubMed:11526337, PubMed:18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed:7721895).
Alternative names
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
What's included?
Recommended products
Human IL-4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IL-4 in Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 6.25 - 400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1.08 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 5 | mean - | SD - | C.V. 6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 91 - 102 % |
Sample type EDTA Plasma | Average % = 109 | Range 103 - 114 % |
Sample type Heparin Plasma | Average % = 95 | Range 88 - 98 % |
Sample type Citrate plasma | Average % = 100 | Range 94 - 104 % |
Sample type Cell culture media | Average % = 117 | Range 112 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL-4 ELISA Kit (ab215089) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-4 protein in cit plasma, edta plasma, hep plasma, plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-4 with 1.08 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL-4 is a 129 amino acids long secreted protein. IL-4 is a cytokine produced mostly by activated T lymphocytes, mast cells and basophils. It has variety of immune response modulating functions by acting on number of cell types. IL-4 participates in several B-cell activation processes. It is a co-stimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 positively regulates IL31RA expression in macrophages.
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6 product images
Sandwich ELISA - Human IL-4 ELISA Kit (ab215089)
Human IL-4 standard curve comparison data.
Standard curve comparison between human IL-4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 8-fold increase in sensitivity.
Sandwich ELISA - Human IL-4 ELISA Kit (ab215089)
Example of human IL-4 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-4 ELISA Kit (ab215089)
Example of human IL-4 standard curve in Sample Diluent 75BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL-4 ELISA Kit (ab215089)
Interpolated concentrations of native IL-4 in PHA-M stimulated human PBMC cell culture supernatant samples.
The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated human PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 174.6 pg/mL in stimulated human PBMC cell culture supernatant.
Sandwich ELISA - Human IL-4 ELISA Kit (ab215089)
Interpolated concentrations of spike recombinant IL-4 in serum and plasma samples.
The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma citrate 100%, plasma EDTA 100%, plasma heparin 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human IL-4 ELISA Kit (ab215089)
Comparison of IL-4 in unstimulated and PHA-M stimulated human PBMC cell supernatants.
Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of Il-4 were measured in neat supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 174.6 pg/mL in PHA-M stimulated PBMC cell supernatant, 1.4 pg/mL in unstimulated supernatants and undetectable in media (not shown).
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