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AB46058

Human IL-4 ELISA Kit

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(1 Publication)

Human IL-4 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-4 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

1 Images
Sandwich ELISA - Human IL-4 ELISA Kit (AB46058)
  • sELISA

Unknown

Sandwich ELISA - Human IL-4 ELISA Kit (AB46058)

Representative standard curve using ab46058

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 0.7 pg/mL

Range

1.1 - 35 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-4 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in supernatants, buffered solutions, serum and plasma samples.

A monoclonal antibody specific IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL4 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-4.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" } ]
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Recovery

[ { "sample": "Serum", "range": "75 - 121 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46058-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46058-CMP04" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46058-CMP10" }, { "size":"1 x 400 µL", "name":"Biotinylated anti IL-4", "number":"AB46058-CMP02" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46058-CMP05" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46058-CMP09" }, { "size":"2 x 1 Vial", "name":"IL-4 Standard (Lyophilized)", "number":"AB46058-CMP12" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46058-CMP06" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46058-CMP07" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46058-CMP08" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46058-CMP01" }, { "size":"1 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46058-CMP11" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46058-CMP03" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46058-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46058-CMP04", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46058-CMP10", "productcode":"" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46058-CMP08", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46058-CMP03", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46058-CMP09", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46058-CMP01", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti IL-4", "number":"AB46058-CMP02", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46058-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46058-CMP07", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-4 Standard (Lyophilized)", "number":"AB46058-CMP12", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46058-CMP11", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46058-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of functional biomaterials 15: PubMed39194653

2024

Osteogenic Protection against Fine Dust with Erucic Acid-Induced Exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunjung Kim,Boyong Kim
View all publications
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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