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AB326012

Human IL-4 ELISA Kit, Chemiluminescent

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Human IL-4 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IL-4 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.61 - 1333.33 pg/ml with a sensitivity of 0.39 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

5 Images
Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)
  • sELISA

Lab

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)
  • sELISA

Lab

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)

Interpolated concentration of native IL-4 was measured in duplicate at different sample concentrations. Undiluted samples are 100% stimulated PBMC supernatant from cells grown in the presence of 1.5% PHA-M for 46 hours. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)
  • sELISA

Lab

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)

Human PBMC cells were cultured in the absence and presence of 1.5% PHA-M for 46 hours. The concentrations of Il-4 were measured in neat supernatant samples in duplicates and interpolated from the IL-4 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean IL-4 concentration was determined to be 174.6 pg/mL in PHA-M stimulated PBMC cell supernatant, 1.4 pg/mL in unstimulated supernatants and undetectable in media (not shown).

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)
  • sELISA

Lab

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)

Dynamic range comparison between the Chemiluminescent Human IL-4 SimpleStep ELISA, colorimetric Human IL-4 SimpleStep ELISA (ab215089), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and range.

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)
  • sELISA

Lab

Sandwich ELISA - Human IL-4 ELISA Kit, Chemiluminescent (AB326012)

Standard curve comparison between the Chemiluminescent Human IL-4 SimpleStep ELISA and colorimetric Human IL-4 SimpleStep ELISA (ab215089) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.39 pg/mL

Range

0.61 - 1333.33 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-4 ELISA Kit, Chemiluminescent (ab326012) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-4 in Cell Culture Supernatant, Cell Culture Media with a sensitivity of 0.39 pg/mL.

How the assay works

Human IL-4 ELISA Kit, Chemiluminescent (ab326012) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-4 (ab326012) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-4 antibody [EPR19760-240] BSA and Azide free (Capture) ab242991
- Anti-IL-4 antibody [EPR19760-17] BSA and Azide free (Detector) ab242991
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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "112 - 120 %", "average": "= 117" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326012-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326012-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-4 Capture Antibody", "number":"AB326012-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326012-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326012-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326012-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326012-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-4 Lyophilized Recombinant Protein", "number":"AB326012-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326012-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-4 Detector Antibody", "number":"AB326012-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB326012-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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