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AB46063

Human IL-4 ELISA Kit High Sensitivity

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(3 Publications)

Human IL-4 ELISA Kit High Sensitivity is a Sandwich (quantitative) ELISA for the measurement of Human IL-4 High Sensitivity in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

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ELISA - Human IL-4 ELISA Kit High Sensitivity (AB46063)
  • ELISA

Lab

ELISA - Human IL-4 ELISA Kit High Sensitivity (AB46063)

IL-4 measured in cell culture supernatants from control (PBMC) or treated (PBMC+PHA; 2% PHA-M; LifeTechnologies) cells, grown for 48 hours (duplicates +/- SD). Samples tested neat - 1/10, control supernatant gave signals below level of detection.

ELISA - Human IL-4 ELISA Kit High Sensitivity (AB46063)
  • ELISA

Lab

ELISA - Human IL-4 ELISA Kit High Sensitivity (AB46063)

Standard curve of human IL-4, with background signal subtracted (duplicates; +/- SD).

Sandwich ELISA - Human IL-4 ELISA Kit High Sensitivity (AB46063)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4 ELISA Kit High Sensitivity (AB46063)

Representative Standard Curve using ab46063

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.31 pg/mL

Range

0.31 - 10 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-4 Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-4 in Human serum, plasma, buffered solution and cell culture media.

A monoclonal antibody specific for IL-4 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-4.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample E", "replicates": 7, "mean": "2 pg/mL", "standardDeviation": "0.874", "coefficientOfVariability": "17" }, { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 9, "mean": "7.1 pg/mL", "standardDeviation": "0.83", "coefficientOfVariability": "11.8" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 9, "mean": "6.47 pg/mL", "standardDeviation": "0.72", "coefficientOfVariability": "11.1" }, { "reproducibilityType": "Inter", "sample": "Sample C", "replicates": 9, "mean": "8.76 pg/mL", "standardDeviation": "1.37", "coefficientOfVariability": "15.7" }, { "reproducibilityType": "Inter", "sample": "Sample D", "replicates": 9, "mean": "7.44 pg/mL", "standardDeviation": "1.2", "coefficientOfVariability": "16.1" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "7.43 pg/mL", "standardDeviation": "0.9", "coefficientOfVariability": "12.3" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "6.2 pg/mL", "standardDeviation": "0.7", "coefficientOfVariability": "10.8" }, { "reproducibilityType": "Intra", "sample": "Sample C", "replicates": 8, "mean": "8.77 pg/mL", "standardDeviation": "0.7", "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Intra", "sample": "Sample D", "replicates": 8, "mean": "7.36 pg/mL", "standardDeviation": "0.4", "coefficientOfVariability": "6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 127 %", "average": "= 95" }, { "sample": "Serum", "range": "61 - 75 %", "average": "= 69" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46063-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"Amplification Diluent", "number":"AB46063-CMP12", "productcode":"" }, { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46063-CMP03", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti IL-4", "number":"AB46063-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-4 Microplate (12 x 8 well strips)", "number":"AB46063-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46063-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-4 Standard (Lyophilized)", "number":"AB46063-CMP10", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46063-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46063-CMP06", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46063-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46063-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"Amplifier", "number":"AB46063-CMP11", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46063-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39795937

2025

Effective Targeting of Glutamine Synthetase with Amino Acid Analogs as a Novel Therapeutic Approach in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shimaa Abdelsattar,Hiba S Al-Amodi,Hala F Kamel,Ahood A Al-Eidan,Marwa M Mahfouz,Kareem El Khashab,Amany M Elshamy,Mohamed S Basiouny,Mohamed A Khalil,Khaled A Elawdan,Shorouk Elsaka,Salwa E Mohamed,Hany Khalil

International journal of biological sciences 16:994-1009 PubMed32140068

2020

DNA methylation is involved in the pathogenesis of osteoarthritis by regulating expression and CtBP-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangxiang Sun,Lin Xiao,Juan Chen,Xun Chen,Xinlin Chen,Shuxin Yao,Hui Li,Guanghui Zhao,Jianbing Ma

The American journal of pathology 190:674-688 PubMed31972160

2020

Inflammation and DNA Methylation-Dependent Down-Regulation of miR-34b-5p Mediates c-MYC Expression and CRL4 E3 Ligase Activity in Colitis-Associated Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Yang,Wenzhu Lu,Hongbo He,Hong Liu
View all publications
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