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AB46022

Human IL-4 Receptor ELISA Kit

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(2 Publications)

Human IL-4 Receptor ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-4 Receptor in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

1 Images
Sandwich ELISA - Human IL-4 Receptor ELISA Kit (AB46022)
  • sELISA

Unknown

Sandwich ELISA - Human IL-4 Receptor ELISA Kit (AB46022)

Representative Standard Curve using ab46022

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 11.5 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

1h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human IL-4 Receptor (IL-4R) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-4 receptor molecule (CD124) in Human serum, plasma, buffered solutions or cell culture medium.

A monoclonal antibody specific for IL-4R has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-4R concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-4R are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-4R present in the samples.

This kit will recognize both endogenous and recombinant Human IL-4R.

The standard is affinity purified.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "530.5 pg/mL", "standardDeviation": "35.1", "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "106.5 pg/mL", "standardDeviation": "7.58", "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "563.7 pg/mL", "standardDeviation": "17.8", "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "140.6 pg/mL", "standardDeviation": "9.6", "coefficientOfVariability": "6.8" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46022-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46022-CMP04" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46022-CMP02" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46022-CMP03" }, { "size":"4 x 1 Vial", "name":"IL-4R Standard (Lyophilized)", "number":"AB46022-CMP09" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46022-CMP06" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46022-CMP10" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46022-CMP07" }, { "size":"2 x 1 Unit", "name":"IL-4R Microplate (12 x 8 well strips)", "number":"AB46022-CMP08" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46022-CMP05" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-IL-4R", "number":"AB46022-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46022-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46022-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46022-CMP10", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46022-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-4R Microplate (12 x 8 well strips)", "number":"AB46022-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46022-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-4R Standard (Lyophilized)", "number":"AB46022-CMP09", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46022-CMP02", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46022-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46022-CMP07", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-IL-4R", "number":"AB46022-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
See full target information IL4R
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37040185

2023

Cytokine storm-based mechanisms for extrapulmonary manifestations of SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Del Nogal Avila,Ranjan Das,Joubert Kharlyngdoh,Eduardo Molina-Jijon,Hector Donoro Blazquez,Stéphanie Gambut,Michael Crowley,David K Crossman,Rasheed A Gbadegesin,Sunveer S Chugh,Sunjeet S Chugh,Carmen Avila-Casado,Camille Macé,Lionel C Clement,Sumant S Chugh

Experimental and therapeutic medicine 19:301-307 PubMed31853303

2019

Endoscopic removal of nucleus pulposus of intervertebral disc on lumbar intervertebral disc protrusion and the influence on inflammatory factors and immune function.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Xu,Changchun Zhang,Kun Zhu,Zhengqi Bao,Pinghui Zhou,Xiaojun Li
View all publications
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websiteProtocolBooklet
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