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AB316898

Human IL-4I1/LAO ELISA Kit- Extracellular

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Human IL-4I1/LAO ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Human IL-4I1/LAO with a sensitivity of 43.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

FIG1, UNQ636/PRO1265, IL4I1, L-amino-acid oxidase, LAAO, LAO, Interleukin-4-induced protein 1, Protein Fig-1, IL4-induced protein 1, hIL4I1, hFIG1

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Sandwich ELISA - Human IL-4I1/LAO ELISA Kit- Extracellular (AB316898)
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Supplier Data

Sandwich ELISA - Human IL-4I1/LAO ELISA Kit- Extracellular (AB316898)

Example of human IL4I1/LAO standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-4I1/LAO ELISA Kit- Extracellular (AB316898)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4I1/LAO ELISA Kit- Extracellular (AB316898)

Interpolated concentration of native IL4I1/LAO was measured in duplicate at different sample concentrations. Undiluted samples are 7.5% treated (24 hrs 50 nM PMA, then 24 hrs 5 µg/ml LPS) THP-1 cell culture supernatant and mock treated THP-1 cell culture supernatant.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. Interpolated concentrations of IL4I1 in mock-treated THP-1 cell culture supernatant were below the detectable range of the assay.

Sandwich ELISA - Human IL-4I1/LAO ELISA Kit- Extracellular (AB316898)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4I1/LAO ELISA Kit- Extracellular (AB316898)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL4I1/LAO concentration of detected samples was determined to be 1,251 pg/mL with a range of undetected – 1,251 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 43.6 pg/mL

Range

109.375 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB222102

Anti-IL-4I1/LAO antibody [EPR22070]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-IL-4I1/LAO antibody [EPR22070] (AB222102)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-4I1/LAO SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-4I1/LAO protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human IL-4I1/LAO with 43.6 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]
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Recovery

[ { "sample": "Serum", "range": "79 - 84 %", "average": "= 81" }, { "sample": "EDTA Plasma", "range": "79 - 98 %", "average": "= 87" }, { "sample": "Citrate plasma", "range": "76 - 86 %", "average": "= 82" }, { "sample": "Heparin Plasma", "range": "81 - 84 %", "average": "= 82" }, { "sample": "Cell culture supernatant", "range": "87 - 105 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Secreted L-amino-acid oxidase that acts as a key immunoregulator (PubMed : 17356132, PubMed : 32818467, PubMed : 32866000). Has preference for L-aromatic amino acids : converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively (PubMed : 17356132, PubMed : 32818467, PubMed : 32866000). Also has weak L-arginine oxidase activity (PubMed : 26673964). Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR (PubMed : 32818467, PubMed : 32866000). IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer (PubMed : 32818467, PubMed : 32866000). AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity (PubMed : 32818467). Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation : inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells (PubMed : 17356132, PubMed : 25446972, PubMed : 25778793, PubMed : 28891065). Also regulates M2 macrophage polarization by inhibiting T-cell activation (By similarity). Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2 (PubMed : 23355881).
See full target information IL4I1
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websiteProtocolBooklet
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Product promise

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