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AB243668

Human IL-4R ELISA Kit

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(1 Publication)

Human IL-4R ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-4R with a sensitivity of 1.44 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

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ELISA - Human IL-4R ELISA Kit (AB243668)
  • ELISA

Supplier Data

ELISA - Human IL-4R ELISA Kit (AB243668)
Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)

25% serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4R concentration was determined to be 687.54 pg/mL with a range of 318.76 – 1223.19 pg/mL.

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)

25% serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4R concentration was determined to be 1026.45 pg/mL with a range of 689.71 – 1565.79 pg/mL.

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)

Interpolated concentrations of native IL-4R in human serum and plasma samples.

The concentrations of IL-4R were measured in duplicates, interpolated from the IL-4R standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50% and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-4R concentration was determined to be 974.35 pg/mL in serum, 835.80 pg/mL in plasma (citrate) and 970.16 pg/mL in plasma (EDTA), and 932.74 pg/mL in plasma (heparin).

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-4R ELISA Kit (AB243668)

Example of human IL-4R standard curve in Sample Diluent NS.

The IL-4R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.44 pg/mL

Range

7.81 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-4R ELISA Kit ab243668 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-4R in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.44 pg/mL.

How the assay works

Human IL-4R SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-4R ELISA Kit ab243668 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-4R antibody [EPR21947-225] - BSA and Azide free (Capture) ab259498
- Anti-IL-4R antibody [EPR21947-278] - BSA and Azide free (Detector) ab259498
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "91 - 99 %", "average": "= 95" }, { "sample": "Serum", "range": "91 - 94 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "98 - 106 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "103 - 112 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "87 - 97 %", "average": "= 92" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243668-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243668-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243668-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243668-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243668-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243668-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-4R Capture Antibody", "number":"AB243668-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB243668-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-4R Lyophilized Recombinant Protein", "number":"AB243668-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243668-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-4R Detector Antibody", "number":"AB243668-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
See full target information IL4R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:628512 PubMed33868246

2021

RNA-Seq Identifies Marked Th17 Cell Activation and Altered CFTR Expression in Different Atopic Dermatitis Subtypes in Chinese Han Populations.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tian,Baoyi Liu,Lijie Chen,Yongyi Xie,Jingyao Liang,Yan Yang,Lei Shao,Jing Zhang,Jianqin Wang,Xibao Zhang,Zhouwei Wu,Yumei Liu
View all publications
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