Human IL-5 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-5 in Human in Plasma, Cell culture supernatant, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.74 - 2000 pg/mL
- Sensitivity
- < 5 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).
Alternative names
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
What's included?
Recommended products
Human IL-5 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-5 in Human in Plasma, Cell culture supernatant, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.74 - 2000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 5 pg/mL
- General recovery
- 95%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 94.83 | Range 84 - 103 % |
Sample type Serum | Average % = 95.46 | Range 83 - 103 % |
Sample type Plasma | Average % = 96.32 | Range 84 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's IL-5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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2 product images
Sandwich ELISA - Human IL-5 ELISA Kit (ab100571)
Representative standard curve using ab100571
Sandwich ELISA - Human IL-5 ELISA Kit (ab100571)
Representative standard curve using ab100571
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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