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AB215536

Human IL-5 ELISA Kit

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(3 Publications)

Human IL-5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-5 with a sensitivity of 3.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5

5 Images
ELISA - Human IL-5 ELISA Kit (AB215536)
  • ELISA

Supplier Data

ELISA - Human IL-5 ELISA Kit (AB215536)
Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)
  • sELISA

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Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)

Human IL-5 standard curve comparison data.

Standard curve comparison between human IL-5 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)

Interpolated concentrations of spiked IL-5 in human serum, plasma and urine samples.

The concentrations of IL-5 were measured in duplicates, interpolated from the IL-5 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 149 pg/mL in serum, 131 pg/mL in plasma (citrate), 165 pg/mL in plasma (heparin), 142 pg/mL in plasma (EDTA) and 133 pg/mL in urine.

Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)

Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.

Conditioned media was harvested after 48 hours. IL-5 was measured in 100% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-5 were measured in duplicate, interpolated from the IL-5 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 concentration was determined to be 226.9 pg/mL in PHA stimulated PBMC supernatant and 1.94 pg/mL in unstimulated PBMC supernatant.

Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-5 ELISA Kit (AB215536)

Example of human IL-5 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.3 pg/mL

Range

4.7 - 300 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IL-5 ELISA Kit ab215536 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-5 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 3.3 pg/mL.

How the assay works

Human IL-5 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-5 ELISA Kit ab215536 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) ab242805
- Anti-IL-5 antibody [EPR20012-146] - BSA and Azide free (Detector) ab242805

Interleukin 5 (IL-5) is a small, 113 amino acid cytokine produced by T cells involved in the differentiation of B-cells to immunoglobulin secreting cells. IL-5 is a key mediator in the survival and effector functions of mature eosinophils. Due to its effects on eosinophils, IL-5 is implicated in the pathogenesis of asthma and other hypereosinophilic inflammatory conditions.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "104 - 107 %", "average": "= 105" }, { "sample": "Serum", "range": "93 - 100 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "93 - 96 %", "average": "= 94" }, { "sample": "Urine", "range": "80 - 81 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "97 - 100 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "90 - 96 %", "average": "= 93" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 2653458, PubMed : 9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed : 1495999, PubMed : 22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed : 7613138).
See full target information IL5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed39940325

2025

Immature Sword Bean () Pod Alleviates Allergic Rhinitis (A Double-Blind Trial) Through PI3K/Akt/mTOR Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Jeong Hwang,Hyeock Yoon,Joo-Hyung Cho,Seong Lee,Kyung-A Hwang,Young Jun Kim

World journal of gastrointestinal oncology 16:3118-3157 PubMed39072171

2024

Kombo knife combined with sorafenib in liver cancer treatment: Efficacy and safety under immune function influence.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Cao,Pei-Pei Li,Bing-Li Qiao,Quan-Wang Li

Modern rheumatology 28:1004-1008 PubMed29385874

2018

Stage classification of IgG4-related dacryoadenitis and sialadenitis by the serum cytokine environment.

Applications

Unspecified application

Species

Unspecified reactive species

Motohisa Yamamoto,Kenichi Takano,Ryuta Kamekura,Chisako Suzuki,Shingo Ichimiya,Tetsuo Himi,Hiroshi Nakase,Hiroki Takahashi
View all publications
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