AB283549

Human IL-5 Receptor alpha ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human IL-5 Receptor alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-5 Receptor alpha with a sensitivity of 20.55 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125

3 Images

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

sELISA

Supplier Data

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

Interpolated concentrations of native IL-5 Receptor alpha in human serum and plasma samples.

The concentrations of IL-5 Receptor alpha were measured in duplicates, interpolated from the IL-5 Receptor alpha standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 Receptor alpha concentration was determined to be 3,175.24 pg/mL in serum, 2,926.77 pg/mL in plasma (citrate), 3,031.12 pg/mL in plasma (heparin), and 2,817.08 pg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-5 Receptor alpha concentration was determined to be 2,393.78 pg/mL with a range of 1,175.72 – 4,564.80 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

Example of human IL-5 Receptor alpha standard curve in Sample Diluent NS.

The IL-5 Receptor alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 20.55 pg/mL

Range

56.25 - 3600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human IL-5 Receptor alpha ELISA kit (ab283549) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-5 Receptor alpha protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-5 Receptor alpha with 20.55 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Human IL-5 Receptor alpha is a heterodimeric cytokine receptor. Binding of IL-5 activates the beta subunit. It is expressed on eosinophils and basophils and is a target of the drug benralizumab, which leads to almost complete depletion of eosinophils. Benralizumab treatment has been demonstrated in clinical trials to reduce asthma reactions.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "98 - 103 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "99 - 114 %", "average": "= 107" }, { "sample": "Cell culture media", "range": "100 - 103 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "87 - 95 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "87 - 118 %", "average": "= 99" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283549-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB283549-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB283549-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB283549-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB283549-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB283549-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL-5 Receptor alpha Lyophilized Recombinant Protein", "number":"AB283549-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-5 Receptor alpha Capture Antibody", "number":"AB283549-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB283549-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB283549-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IL-5 Receptor alpha Detector Antibody", "number":"AB283549-CMP08", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-5RA also known as the interleukin-5 receptor alpha chain or IL-5 receptor is a protein with a critical role in the immune system. It is also referred to as CD125 or CaL40. The protein has an approximate mass of 52 kDa and is expressed mainly on eosinophils and basophils which are types of white blood cells involved in immune responses. The IL-5RA forms a part of the IL-5 receptor complex which also includes a beta chain responsible for signal transduction.

Biological function summary

IL-5RA plays an important role in the growth differentiation and activation of eosinophils. The protein forms part of the IL-5 receptor complex which binds to the cytokine IL-5 initiating a signaling cascade that results in these processes. Through its interaction with IL-5 IL-5RA influences the survival and function of eosinophils cells critical for responses against parasitic infections and in allergic reactions.

Pathways

IL-5RA is fundamental in the JAK-STAT signaling pathway. This pathway is significant as it facilitates the transduction of extracellular cytokine signals into cellular responses impacting the growth and activity of eosinophils. The IL-5 receptor complex is associated with other cytokine receptors through this pathway involving proteins such as JAK2 and STAT5 which play essential roles in the activation of downstream signaling events and cellular responses.

IL-5RA is linked with asthma and eosinophilic esophagitis conditions characterized by an abnormal proliferation and activation of eosinophils. In asthma for example the increased activity of IL-5RA leads to elevated levels of eosinophils contributing to airway inflammation and hyperresponsiveness. IL-5RA also connects with other proteins like IL-5 itself serving as a therapeutic target in treatments aimed at regulating excessive eosinophil activity in these disorders.

style

left-column

Product protocols

style

left-column

Target data

Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed : 1495999, PubMed : 22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed : 9516124).

See full target information IL5RA

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-5 Receptor alpha ELISA Kit

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Assay Kits

Assay Kits

Product promise