AB178013

Human IL-6 ELISA Kit

Name: Human IL-6 ELISA Kit (ab178013) | Abcam
Brand: Abcam Limited
SKU: AB178013
Price: 820 USD
Availability: InStock
Rating: 5 (2 reviews)

KO Validated
Recombinant
SimpleStep
What is this?

(2 Reviews)

(211 Publications)

Human IL-6 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-6 (IL-6) with a sensitivity of 1.6 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x 96-well plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility – including CSF
- Cited in over 210 publications

View Alternative Names

IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2

12 Images

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

sELISA

Lab

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Example of Human IL-6 standard curve in Sample Diluent NS in 96-well and 384-well plate.

Example of Human IL-6 standard curve in 96-well and 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Interpolated concentrations of Human IL-6 in human stimulated PBMC supernatant in 96-well and 384-well plates.

Interpolated concentration of native IL-6 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2% Human Stimulated PBMC supernatant treated with 1.5% PHA-M for 46 hrs. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Example of human IL6 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Human IL-6 concentration was interpolated from the IL-6 standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-6 ELISA kit (ab178013). Wild-type and IL-6 knockout A549 cells (ab273751) were assessed in duplicate (n=2). Cells were either treated with 20 ng/mL active recombinant human IL-1 beta protein (ab259387) for 24 h to induce expression of IL-6 or not treated with IL-1 beta. Data are represented as the mean and error bars represent standard deviation.

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Interpolated concentrations of spiked IL6 in human serum and plasma samples.

The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (EDTA) 50%, and plasma (citrate) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Unknown

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Linearity of dilution for spiked recombinant IL-6.

Recombinant IL-6 was spiked into biological samples and diluted in a 2-fold dilution series in Sample Diluent NS. NL= Non linear 50% pooled serum and plasma (EDTA, Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

50% serum from ten individual healthy human male and female donors was measured in duplicate. All values were below the detectable range of the assay.

sELISA

Unknown

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Example of raw data value for of human IL-6 standard curve.

Raw data values are shown in the table.

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Representative image of Human IL-6 ELISA Kit (ab178013)

Components shown from left to right :

- Sample diluent NS

- 10X Human IL-6 Detector Antibody

- Human IL-6 Lyophilised Purified Protein (2 vials)

- Human IL-6 Capture Antibody (Lyophilised)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Interpolated concentrations of native IL6 in human peripheral blood monocyte (PBMC) cell culture supernatant samples.

The concentrations of IL6 were measured in duplicates, interpolated from the IL6 standard curves and corrected for sample dilution. Undiluted samples are as follows : Stimulated PBMC Cell culture supernatant 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL6 concentration was determined to be 60,443 pg/mL in stimulated PBMC cell culture supernatant, and undetectable in unstimulated and media controls.

sELISA

Unknown

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Linearity of dilution for native IL-6.

Native IL-6 was measured in biological samples in a 2- fold dilution series. Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

Human IL6 standard curve comparison data.

Standard curve comparison between the previous Human IL6 SimpleStep ELISA^® kit and the re-developed Human IL6 SimpleStep ELISA^® kit. The re-developed kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.6 pg/mL

Range

7.8 - 500 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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You may be interested in:

AB243973

Human/Monkey IL-6 Antibody Pair - BSA and Azide free

0 Reviews

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human IL-6 ELISA Kit ab178013 is a rapid single-wash 90-min ELISA kit to measure Human IL-6 in cell culture supernatant, serum, plasma, cerebral spinal fluid. This SimpleStep ELISA® sensitivity is 1.6 pg/mL.

How the assay works

Human IL-6 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant Human IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit. Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant Human IL-6Ra was prepared at 50 ng/mL and 500 pg/mL and tested for interference. No interference with was observed.

Human IL-6 ELISA Kit ab178013 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IL-6 ELISA kit ab178013

Human IL-6 ELISA kit ab178013 has been used to assess inflammation in different physio-pathological contexts such as obesity⁽¹⁾, coronary artery disease⁽²⁾, and rheumatoid arthritis⁽³⁾.

References:

⁽¹⁾Shaohong Yu et al. 2023, PMID: 38156278,
⁽²⁾Yongshun W. et al. 2023, PMID:38054817,
⁽³⁾Yaju Tan et al. 2024, PMID: 37997635

Save your precious samples

Our 384-well Human IL-6 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human/Monkey IL-6 Antibody Pair - BSA and Azide free ab243973
- Anti-IL-6 antibody [IL-6-10] - BSA and Azide free (Capture) ab244758
- Anti-IL-6 antibody [IL-6-5] - BSA and Azide free (Detector) ab244886

Related and recommended products

Human IL-6 ELISA kit ab178013 is commonly used to measure IL-6 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human TNF alpha ELISA kit ab181421
- Human IL-8 ELISA kit ab214030
- Human IL-1 beta ELISA kit ab214025

In glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.
Explore our comprehensive range of glioblastoma research solutions here: https://www.abcam.com/en-us/products/product-recommendations/glioblastoma-research-tools

In Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.
Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools

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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]

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Recovery

[ { "sample": "Cell culture media", "range": "97 - 103 %", "average": "= 100" }, { "sample": "Cerebral Spinal Fluid", "range": "87 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "81 - 85 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "80 - 85 %", "average": "= 82" }, { "sample": "Serum", "range": "77 - 84 %", "average": "= 82" } ]

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What's included?

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Properties and storage information

Shipped at conditions

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Target data

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).. IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity).. Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability (PubMed : 12794819, PubMed : 17075861). Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed : 12794819). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed : 20823453). 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis (By similarity). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed : 15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).

See full target information IL6

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Publications (211)

Recent publications for all applications. Explore the full list and refine your search

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 30:e13548 PubMed38174788

2024

Glutamic-acid grafted hyaluronic acid inhibits inflammatory factors via fibroblast and skin model tests.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Ru Jiang,Song Wang,Dong Han,Jian Wei,Ya-Nan Wu,Zhe Liu

Frontiers in nutrition 10:1270435 PubMed38156278

2024

Serum levels of Vanin-2 increase with obesity in relation to inflammation of adipose tissue and may be a predictor of bariatric surgery outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Geng,Dongmei Chen,Yanping Wang,Xingrui Yu,Dan Zuo,Xinlu Lv,Xuelian Zhou,Chengju Hu,Xuesong Yang,Xujue Ma,Wenjing Hu,Jiazhuang Xi,Shaohong Yu

Aging 15:14720-14732 PubMed38054817

2023

Meteorin-like protein elevation post-exercise improved vascular inflammation among coronary artery disease patients by downregulating NLRP3 inflammasome activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjin Liu,Liwei Diao,Weiyi Xia,Xiaoyi Zeng,Wen Li,Jieru Zou,Tiansheng Liu,Xinli Pang,Yongshun Wang

International journal of rheumatic diseases 27:e14976 PubMed37997635

2023

Forsythiaside A exhibits anti-migration and anti-inflammation effects in rheumatoid arthritis in vitro model.

Applications

Unspecified application

Species

Unspecified reactive species

Minhui Su,Dinghua Zhou,Jiamin Huang,Ting Yang,Qi Zhou,Yaju Tan

Nature communications 14:7639 PubMed37993431

2023

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Ye He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun Huang

Experimental and therapeutic medicine 26:575 PubMed38023363

2023

IGF2BP2‑dependent STIM1 inhibition protects against LPS‑induced pneumonia by alleviating endoplasmic reticulum stress and the inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Qigang Dai,Ning Su,Zhihui Liu,Jinxing Hu

Nature biotechnology : PubMed37872410

2023

Scalable genetic screening for regulatory circuits using compressed Perturb-seq.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas Yao,Loic Binan,Jon Bezney,Brooke Simonton,Jahanara Freedman,Chris J Frangieh,Kushal Dey,Kathryn Geiger-Schuller,Basak Eraslan,Alexander Gusev,Aviv Regev,Brian Cleary

Scientific reports 13:17969 PubMed37863977

2023

The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model.

Applications

Unspecified application

Species

Unspecified reactive species

Alexe Grenier,Mathieu C Morissette,Patrick J Rochette,Roxane Pouliot

AMB Express 13:107 PubMed37789186

2023

Bioactive metabolites identified from Aspergillus terreus derived from soil.

Applications

Unspecified application

Species

Unspecified reactive species

Menna Fayek,Hassan Y Ebrahim,Mohamed S Abdel-Aziz,Heba Taha,Fatma A Moharram

In vivo (Athens, Greece) 37:2028-2038 PubMed37652511

2023

Therapeutic Effect of Human Adipocyte-derived Stem Cell-derived Exosomes on a Transgenic Mouse Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lung Chan,Wayne Hsu,Kai-Yun Chen,Weu Wang,Yi-Chieh Hung,Chien-Tai Hong

View all publications

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