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Human IL-6 ELISA Kit High Sensitivity is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-6 High Sensitivity in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, IL6, IFNB2
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.56 - 50 pg/mL
- Assay time
3h 45m
- Sensitivity
< 0.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human IL-6 ELISA Kit High Sensitivity is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-6 High Sensitivity in Human in Plasma, Cell culture supernatant, Serum samples.
Alternative names=Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2, IL6, IFNB2
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.56 - 50 pg/mL
- Assay time
3h 45m
- Assay Platform
Microplate
- Sensitivity
< 0.8 pg/mL
- General recovery
93%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Sample 1 | n 18 | mean 25 pg/mL | SD 3 | C.V. 12.2 |
Sample Sample 2 | n 18 | mean 12 pg/mL | SD 1 | C.V. 8.7 |
Sample Sample 3 | n 18 | mean 6 pg/mL | SD 1 | C.V. 13.7 |
Sample Sample 4 | n 18 | mean 41 pg/mL | SD 2 | C.V. 5.9 |
Sample Sample 5 | n 18 | mean 31 pg/mL | SD 1 | C.V. 4.9 |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human IL-6 ELISA kit - High sensitivity is a sandwich ELISA kit for the quantitative measurement of IL-6 in supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognise both natural and recombinant Human IL-6.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
Get results in 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
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