JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB46029

Human IL-6 Receptor ELISA Kit

4

(1 Review)

|

(6 Publications)

Human IL-6 Receptor ELISA Kit is a Sandwich ELISA for the measurement of Human IL-6 Receptor in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD126, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Membrane glycoprotein 80, gp80, IL6R

1 Images
Sandwich ELISA - Human IL-6 Receptor ELISA Kit (AB46029)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-6 Receptor ELISA Kit (AB46029)

Representative Standard Curve using ab46029

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 5 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

1h 45m

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Assay Kits

AB214025

Human IL-1 beta ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

  • 4
  • 1
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IL-6 Receptor (Interleukin-6 Receptor) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble IL-6 Receptor in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-6 Receptor has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 Receptor concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 Receptor are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 Receptor present in the samples.

This kit will recognize both endogenous and recombinant Human IL-6 Receptor.

Get higher sensitivity in only 90 minutes with Human IL-6 Receptor ELISA Kit (ab181424) from our SimpleStep ELISA® range.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 24, "mean": "983.9 pg/mL", "standardDeviation": "10.46", "coefficientOfVariability": "1.06" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 24, "mean": "136.2 pg/mL", "standardDeviation": "6.086", "coefficientOfVariability": "4.46" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 7, "mean": "977.7 pg/mL", "standardDeviation": "3.04", "coefficientOfVariability": "0.31" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 7, "mean": "131.1 pg/mL", "standardDeviation": "2.489", "coefficientOfVariability": "2.289" } ]
style
left-column

What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46029-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46029-CMP03" }, { "size":"4 x 1 Vial", "name":"IL-6R Standard", "number":"AB46029-CMP11" }, { "size":"4 x 1 Vial", "name":"IL-6R Control", "number":"AB46029-CMP09" }, { "size":"2 x 1 Unit", "name":"IL-6 Receptor Microplate (12 x 8 well strips)", "number":"AB46029-CMP10" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46029-CMP05" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-IL-6R (CD126)", "number":"AB46029-CMP08" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46029-CMP04" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46029-CMP07" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46029-CMP01" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46029-CMP02" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46029-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46029-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46029-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-6R Standard", "number":"AB46029-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-6R Control", "number":"AB46029-CMP09", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46029-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-6 Receptor Microplate (12 x 8 well strips)", "number":"AB46029-CMP10", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-IL-6R (CD126)", "number":"AB46029-CMP08", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46029-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46029-CMP05", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46029-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46029-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46029-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-6R also known as the IL-6 receptor or IL-6R protein is a transmembrane protein with an approximate mass of 80-90 kDa. This receptor is found on the surface of various cells including hepatocytes leukocytes and immune cells like T-cells and macrophages. IL-6R binds to interleukin-6 (IL-6) a cytokine and plays an important role in immune response regulation. The receptor exists in both membrane-bound and soluble forms each contributing to diverse signaling events.
Biological function summary

IL-6 receptor function involves mediating the effects of IL-6 through its interaction with glycoprotein 130 (gp130) forming a signaling complex. This complex initiates intracellular pathways that lead to gene expression changes necessary for immune responses inflammation and hematopoiesis. When IL-6 binds IL-6R the complex formation with gp130 triggers downstream signaling that impacts cell growth differentiation and survival.

Pathways

IL-6R is central to the JAK/STAT signaling pathway one of the two main pathways activated by IL-6 signaling. It's also involved in the MAPK cascade contributing to cellular responses such as proliferation and differentiation. Through these pathways IL-6R interacts with other proteins like JAK kinases and STAT transcription factors coordinating cellular responses to external signals.

IL-6R is associated with inflammatory diseases such as rheumatoid arthritis and certain cancers. In rheumatoid arthritis overexpression of IL-6R leads to persistent inflammation and joint damage. In cancer IL-6/IL-6R signaling promotes tumorigenesis and cancer cell survival through interactions with proteins like STAT3. Understanding these connections aids in developing therapeutic interventions targeting IL-6R in various pathological conditions.
style
left-column

Product protocols

style
left-column

Target data

Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal (PubMed : 28265003). Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis (PubMed : 30995492, PubMed : 31235509). The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).. Isoform 1. Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (By similarity).. Isoform 2. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed : 21990364). The IL6 : sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (PubMed : 21990364). In complex with IL6, is required for induction of VEGF production (PubMed : 12794819). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (By similarity).. Soluble interleukin-6 receptor subunit alpha. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed : 21990364). The IL6 : sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (PubMed : 21990364). In complex with IL6, is required for induction of VEGF production (PubMed : 12794819). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (By similarity).
See full target information IL6R
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 12:95 PubMed35725649

2022

Blockade of the Arid5a/IL-6/STAT3 axis underlies the anti-inflammatory effect of Rbpjl in acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiachen Lv,Min Fang,Shijie Sun,Gang Wang,Songbin Fu,Bei Sun,Jinxue Tong

Frontiers in physiology 12:722528 PubMed34707507

2021

Soluble Interleukin-6 Receptor Regulates Interleukin-6-Dependent Vascular Remodeling in Long-Distance Runners.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Villar-Fincheira,Aaron J Paredes,Tomás Hernández-Díaz,Ignacio Norambuena-Soto,Nicole Cancino-Arenas,Fernanda Sanhueza-Olivares,Felipe Contreras-Briceño,Jorge Mandiola,Nicole Bruneau,Lorena García,María Paz Ocaranza,Rodrigo Troncoso,Luigi Gabrielli,Mario Chiong

Proceedings of the National Academy of Sciences of the United States of America 117:21420-21431 PubMed32817494

2020

The glutathione peroxidase 8 (GPX8)/IL-6/STAT3 axis is essential in maintaining an aggressive breast cancer phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Anees Khatib,Balakrishnan Solaimuthu,Michal Ben Yosef,Areej Abu Rmaileh,Mayur Tanna,Gidi Oren,Michal Schlesinger Frisch,Jonathan H Axelrod,Michal Lichtenstein,Yoav D Shaul

Frontiers in immunology 11:1272 PubMed32733444

2020

IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Zongfei,Chen Rongyi,Cui Xiaomeng,Ma Lili,Ma Lingying,Kong Xiufang,Dai Xiaomin,Zhang Zhuojun,Chen Huiyong,Sun Ying,Jiang Lindi

Frontiers in immunology 9:1789 PubMed30131806

2018

Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Sören Kuypers,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

BMC cancer 15:584 PubMed26268945

2015

Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.

Applications

sELISA

Species

Human

Kingsley O Osuala,Mansoureh Sameni,Seema Shah,Neha Aggarwal,Michelle L Simonait,Omar E Franco,Yan Hong,Simon W Hayward,Fariba Behbod,Raymond R Mattingly,Bonnie F Sloane
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com