Human IL-7 ELISA Kit

Product details

Human IL-7 ELISA Kit ab270218 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-7 in cell culture media, citrate plasma, EDTA plasma, serum. This SimpleStep sensitivity is 5.9 pg/mL.

How the assay works

Human IL-7 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IL-7 ELISA Kit ab270218 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-7 antibody [EPR19464-214] - BSA and Azide free (Capture) ab271310
- Anti-IL-7 antibody [EPR21706-126] - BSA and Azide free (Detector) ab271310

IL-7 (Interleukin 7) is a 20kDa hematopoietic growth factor secreted by stromal cells residing in the bone marrow, thymus, spleen and gut. IL-7 plays a critical role during early T-cell development of naпve and memory CD8+ T cells, as well as during the proliferation and differentiation of B cells. Furthermore, IL-7 has been shown to play a key role in coreceptor reversal, a process which induces the expression of CD8 receptor in a T-cell that has begun differentiation as a CD4+ T cell. IL-7 belongs to a family of short-chain four-helical bundle cytokines. These family members signal through heterodimeric receptor complexes that contain the common cytokine receptor gamma-chain, and activate signaling pathways that promote cell proliferation and survival such as the PI3K, MAPK, and JAK/STAT. IL-7 has been implicated in the development of rheumatoid arthritis (RA), HIV, acute graft-vs-host disease (aGVHD) and X-linked severe combined immunodeficiency (XSCID). In RA, IL-7 activates the production of TNF-alpha by CD4+ T cells and macrophages within the synovial fluid, leading to the inflammation characteristic of RA. In patients with HIV, IL-7 homeostasis is impaired due to decreased expression of the IL-7R. The loss of T-cell homeostasis induces the lymphopenic condition characteristic of an HIV infection. Furthermore, the T-cell cytotoxic regimen prior to bone marrow transplantation induces IL-7 secretion, stimulating growth of residual allo-reactive T cells. These allo-reactive T-cells recognize and attack the transplanted cells and host tissues which lead to the development of aGVHD. Additionally, IL-7 knockout studies in mice have demonstrated that IL-7 is crucial for T cell survival as absence of IL-7 leads to XSCID. The rodent IL-7 proteins have low homology to human IL-7; only 56-57% amino acids sequence identity.