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AB46031

Human IL-7 ELISA Kit

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(1 Publication)

Human IL-7 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-7 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-7, IL-7, IL7

1 Images
Sandwich ELISA - Human IL-7 ELISA Kit (AB46031)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-7 ELISA Kit (AB46031)

Representative Standard Curve using ab46031

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 3 pg/mL

Range

6.25 - 200 pg/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-7 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-7 in Human serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-7 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-7 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-7 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-7 present in the samples.

This kit will recognize both endogenous and recombinant Human IL-7.

Get higher sensitivity in only 90 minutes with Human IL-7 ELISA Kit (ab185984) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 29, "mean": "198.9 pg/mL", "standardDeviation": "3.772", "coefficientOfVariability": "1.89" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 29, "mean": "25.68 pg/mL", "standardDeviation": "1.505", "coefficientOfVariability": "5.84" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "198.7 pg/mL", "standardDeviation": "1.667", "coefficientOfVariability": "0.83" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "28.69 pg/mL", "standardDeviation": "1.118", "coefficientOfVariability": "3.86" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46031-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46031-CMP03" }, { "size":"2 x 1 Vial", "name":"IL-7 standard", "number":"AB46031-CMP07" }, { "size":"2 x 1 Vial", "name":"IL-7 Control", "number":"AB46031-CMP05" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46031-CMP10" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46031-CMP01" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46031-CMP09" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46031-CMP02" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46031-CMP08" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46031-CMP11" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-IL-7", "number":"AB46031-CMP04" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46031-CMP12" }, { "size":"1 x 1 Unit", "name":"IL-7 Microplate (12 x 8 well strip)", "number":"AB46031-CMP06" } ] }, "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46031-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46031-CMP03", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-7 standard", "number":"AB46031-CMP07", "productcode":"" }, { "size":"4 x 1 Vial", "name":"IL-7 Control", "number":"AB46031-CMP05", "productcode":"" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46031-CMP09", "productcode":"" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46031-CMP02", "productcode":"" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46031-CMP08", "productcode":"" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46031-CMP01", "productcode":"" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46031-CMP12", "productcode":"" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46031-CMP10", "productcode":"" }, { "size":"2 x 1 Unit", "name":"IL-7 Microplate (12 x 8 well strip)", "number":"AB46031-CMP06", "productcode":"" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-IL-7", "number":"AB46031-CMP04", "productcode":"" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46031-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary

IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.

Pathways

IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.

IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.
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Product protocols

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Target data

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 25870237, PubMed : 7527823). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG (PubMed : 8128231). Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5 (PubMed : 18523275, PubMed : 20974963).
See full target information IL7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 84:108-19 PubMed26483405

2015

Treatment with Interleukin-7 Restores Host Defense against Pneumocystis in CD4+ T-Lymphocyte-Depleted Mice.

Applications

Unspecified application

Species

Unspecified reactive species

S Ruan,D R Samuelson,B Assouline,M Morre,J E Shellito
View all publications
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websiteProtocolBooklet
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