JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB47357

Human IL-7 ELISA Set (without plates)

Be the first to review this product! Submit a review

|

(0 Publication)

Human IL-7 ELISA Set (without plates) is a Sandwich ELISA for the measurement of Human IL-7 ELISA Set (without plates) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interleukin-7, IL-7, IL7

1 Images
Sandwich ELISA - Human IL-7 ELISA Set (without plates) (AB47357)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-7 ELISA Set (without plates) (AB47357)

IL-7 Standard curve ranging from 6.25 to 200 pg/mL

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

6.25 - 200 pg/mL

Assay time

2h 45m

Assay Platform

Reagents

style
left-column

Complementary Products

Assay Kits

AB218266

Human IL-15 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-15 ELISA Kit (AB218266)

  • 0
  • 0
Assay Kits

AB270883

Human IL-2 ELISA Kit

SimpleStep

ELISA - Human IL-2 ELISA Kit (AB270883)

  • 0
  • 0
Assay Kits

AB234569

Human CD4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD4 ELISA Kit (AB234569)

  • 0
  • 0
Assay Kits

AB215536

Human IL-5 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-5 ELISA Kit (AB215536)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IL-7 Human in vitro ELISA Set is designed for the quantitative measurement of IL-7 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

This ELISA Set is intended to measure human IL-7 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

These reagents are intended for research use only, not for diagnostic purposes.

The IL-7 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant human IL-7.

style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab47357-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Vial", "name":"IL-7 standard", "number":"AB47357-CMP01" }, { "size":"2 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB47357-CMP02" }, { "size":"4 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB47357-CMP04" }, { "size":"2 x 0.55 mL", "name":"Detection biotinylated anti-IL-7 antibody", "number":"AB47357-CMP05" }, { "size":"2 x 0.25 mL", "name":"IL-7 Capture antibody", "number":"AB47357-CMP03" } ] }, "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab47357-5x96Tests", "assetComponentDetails": [ { "size":"5 x 1 Vial", "name":"IL-7 standard", "number":"AB47357-CMP01" }, { "size":"1 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB47357-CMP02" }, { "size":"2 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB47357-CMP04" }, { "size":"1 x 0.55 mL", "name":"Detection biotinylated anti-IL-7 antibody", "number":"AB47357-CMP05" }, { "size":"1 x 0.25 mL", "name":"IL-7 Capture antibody", "number":"AB47357-CMP03" } ] }, "15x96Tests": { "sellingSize": "15 x 96 Tests", "publicAssetCode":"ab47357-15x96Tests", "assetComponentDetails": [ { "size":"15 x 1 Vial", "name":"IL-7 standard", "number":"AB47357-CMP01", "productcode":"" }, { "size":"6 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB47357-CMP04", "productcode":"" }, { "size":"3 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB47357-CMP02", "productcode":"" }, { "size":"3 x 0.55 mL", "name":"Detection biotinylated anti-IL-7 antibody", "number":"AB47357-CMP05", "productcode":"" }, { "size":"3 x 0.25 mL", "name":"IL-7 Capture antibody", "number":"AB47357-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Interleukin-7 (IL7) also known as IL-7 is a cytokine with a molecular mass of approximately 20 kDa. This protein plays an important mechanical role in cell signaling. IL7 is primarily expressed in stromal epithelial cells in the thymus and bone marrow as well as in other locations such as the liver and skin. The cytokine interacts with the IL7 receptor a heterodimer consisting of the IL7Rα chain and the common gamma chain (γc). This interaction facilitates various downstream signal transductions essential for immune cell development and homeostasis.
Biological function summary

IL7 is essential for the survival and proliferation of lymphoid cells. It acts on both T cells and B cells critical components of the adaptive immune system. The protein supports the survival of naïve and memory T cells playing a significant role in T cell homeostasis. IL7 does not operate as part of a large complex but interacts directly with its receptor to exert its function. Through its influence on T and B cell proliferation the cytokine sustains lymphoid system functionality and integrity.

Pathways

IL7 plays a pivotal role in the JAK/STAT signaling pathway which is central to cellular responses to cytokines. When IL7 binds to its receptor JAK kinases activate and promote the phosphorylation of STAT5 leading to gene transcription necessary for cell survival and proliferation. Another important pathway is the PI3K/AKT signaling cascade where the cytokine supports cell survival through anti-apoptotic signals. Through these pathways IL7 is interconnected with other cytokines such as IL2 that also function through shared or similar signaling cascades contributing to immune homeostasis.

IL7 has connections with conditions like severe combined immunodeficiency (SCID) and multiple sclerosis (MS). These involve abnormal IL7 signaling or receptor mutations that affect immune cell function. In SCID mutations in the IL7 receptor result in improper T cell development leading to severe immunodeficiency. In MS altered IL7 levels may contribute to the pathogenesis by affecting the survival and activity of autoreactive T cells. Other proteins like IL2R are also involved in these conditions as they share signaling components with IL7 and their dysregulation can lead to similar immune system disturbances.
style
left-column

Product protocols

style
left-column

Target data

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 25870237, PubMed : 7527823). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG (PubMed : 8128231). Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5 (PubMed : 18523275, PubMed : 20974963).
See full target information IL7
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com