AB100575

Human IL-8 ELISA Kit

Name: Human IL-8 ELISA Kit (ab100575) | Abcam
Brand: Abcam Limited
SKU: AB100575

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(8 Publications)

Human IL-8 ELISA Kit is a Sandwich ELISA for the measurement of Human IL-8 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

2 Images

Sandwich ELISA - Human IL-8 ELISA Kit (AB100575)

sELISA

Unknown

Sandwich ELISA - Human IL-8 ELISA Kit (AB100575)

Representative standard curve using ab100575

sELISA

Unknown

Sandwich ELISA - Human IL-8 ELISA Kit (AB100575)

Representative standard curve using ab100575

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 1 pg/mL

Range

0.8 - 600 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-8 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-8 in serum, plasma, and cell culture supernates.

This assay employs an antibody specific for Human IL-8 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL- 8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 106 %", "average": "= 96.18" }, { "sample": "Serum", "range": "84 - 107 %", "average": "= 95.34" }, { "sample": "Plasma", "range": "83 - 104 %", "average": "= 93.85" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100575-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100575-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100575-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-8 Microplate (12 x 8 wells)", "number":"AB100575-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100575-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100575-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100575-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100575-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IL-8 Standard (lyophilized)", "number":"AB100575-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IL-8", "number":"AB100575-CMP09", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.

Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

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Product protocols

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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).

See full target information CXCL8

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40227199

2025

Impact of Glucose, Inflammation and Phytochemicals on , and Glucose Transporter Gene Expression in Human Intestinal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rizliya Visvanathan,Michael J Houghton,Gary Williamson

Journal, genetic engineering & biotechnology 22:100339 PubMed38494270

2024

Phytocompounds-based therapeutic approach: Investigating curcumin and green tea extracts on MCF-7 breast cancer cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Radwa M Fawzy,Amal A Abdel-Aziz,Khalid Bassiouny,Aysam M Fayed

Atherosclerosis 362:11-22 PubMed36435092

2022

Trans-interaction of risk loci 6p24.1 and 10q11.21 is associated with endothelial damage in coronary artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Yi Tay,Kan Xing Wu,Florence Wen Jing Chioh,Matias Ilmari Autio,Nicole Min Qian Pek,Balakrishnan Chakrapani Narmada,Sock-Hwee Tan,Adrian Fatt-Hoe Low,Michelle Mulan Lian,Elaine Guo Yan Chew,Hwee Hui Lau,Shih Ling Kao,Adrian Kee Keong Teo,Jia Nee Foo,Roger Sik Yin Foo,Chew Kiat Heng,Mark Yan Yee Chan,Christine Cheung

Nanomaterials (Basel, Switzerland) 10: PubMed33266223

2020

Impedimetric Detection of Femtomolar Levels of Interleukin6, Interleukin 8, and Tumor Necrosis Factor Alpha Based on Thermally Modified Nanotubular Titanium Dioxide Arrays.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Arkusz,Ewa Paradowska

Frontiers in oncology 9:1220 PubMed31781509

2019

Selective Regulation of B-Raf Dependent K-Ras/Mitogen-Activated Protein by Natural Occurring Multi-kinase Inhibitors in Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed I Abd El Maksoud,Rehab F Taher,Ahmed H Gaara,Eman Abdelrazik,Omar S Keshk,Khaled A Elawdan,Salwa E Morsy,Ahmed Salah,Hany Khalil

Turkish journal of haematology : official journal 34:254-257 PubMed28148470

2017

Diagnostic Accuracy of Interleukin-6, Interleukin-8, and Interleukin-10 for Predicting Bacteremia in Children with Febrile Neutropenia.

Applications

Unspecified application

Species

Unspecified reactive species

Zümrüt Şahbudak Bal,Nihal Karadaş Özdemir,Semra Şen,Deniz Yılmaz Karapınar,Elif Azarsız,Şöhret Aydemir,Fadıl Vardar

Oncotarget 7:31663-80 PubMed27203220

2016

microRNA-7-5p inhibits melanoma cell proliferation and metastasis by suppressing RelA/NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Keith M Giles,Rikki A M Brown,Clarissa Ganda,Melissa J Podgorny,Patrick A Candy,Larissa C Wintle,Kirsty L Richardson,Felicity C Kalinowski,Lisa M Stuart,Michael R Epis,Nikolas K Haass,Meenhard Herlyn,Peter J Leedman

Molecular cancer therapeutics 12:2541-58 PubMed24026012

2013

Axl mediates acquired resistance of head and neck cancer cells to the epidermal growth factor receptor inhibitor erlotinib.

Applications

Unspecified application

Species

Unspecified reactive species

Keith M Giles,Felicity C Kalinowski,Patrick A Candy,Michael R Epis,Priscilla M Zhang,Andrew D Redfern,Lisa M Stuart,Gregory J Goodall,Peter J Leedman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IL-8 ELISA Kit

Sandwich ELISA - Human IL-8 ELISA Kit (AB100575)

Sandwich ELISA - Human IL-8 ELISA Kit (AB100575)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise