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AB214030

Human IL-8 ELISA Kit

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(2 Reviews)

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(34 Publications)

Human IL-8 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-8 (IL-8) with a sensitivity of 1.8 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for serum, plasma, cell culture supernatants, and cerebrospinal fluid
- Cited in over 30 publications

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

12 Images
Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Example of human IL-8 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Example of human IL-8 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.25% stimulated PBMC supernatant. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 61.6 pg/mL with a range of 62 – 174.9 pg/mL; two individuals (Donor #3 and Donor #9) measured below the minimal detectable dose.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Human IL-8 standard curve comparison data.

Standard curve comparison between human IL-8 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA® kit shows a 3-fold increase in sensitivity.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Human IL-8 concentration was interpolated from the standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human IL-8 ELISA kit (ab214030). Wild-type PC-3 cells and CXCL8 knockout PC-3 cells (ab273743) were assessed in duplicate (n=2) and were either treated with 2 μg/ml LPS for 6 hours to induce expression of IL-8 or not treated with LPS. Data are represented as the mean and error bars represent standard deviation.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-8 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Interpolated concentrations of native IL-8 in human serum.

The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-8 concentration was determined to be 63.2 pg/mL in serum.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Interpolated concentrations of native IL-8 in human PBMC cell culture supernatant.

The concentrations of IL-8 were measured in duplicates, interpolated from the IL-8 standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated 1 : 400 and stimulated 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Measured values were interpolated from the IL-8 Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed. The mean IL-8 concentration was determined to be 2.2 ng/mL in unstimulated, 119.7ng/mL in stimulated, and undetectable in media.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IL-8 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB214030)

Example of human IL-8 standard curve in Sample Diluent NS.

The IL-8 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.8 pg/mL

Range

3.91 - 250 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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You may be interested in:

AB241839

Human/Monkey IL-8 Antibody Pair - BSA and Azide free

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0 Reviews

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Human IL-8 ELISA Kit ab214030 is a rapid single-wash 90-min ELISA kit to measure Human IL-8 in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 1.8 pg/mL.

How the assay works
Human IL-8 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit recognizes both native and recombinant human IL-8 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Human IL-8 ELISA Kit ab214030 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IL-8 ELISA Kit ab214030
Human IL-8 ELISA Kit ab214030 has been used to assess inflammation in different physio-pathological contexts such as traumatic brain injury(1), pulmonary disease(2), and osteoarthritis(3).

References:
(1)Jindan Gao et al. 2022, PMID: 36247290,
(2)Rui Zheng et al. 2022, PMID: 35191419,
(3)Zhi Lv et al. 2021, PMID: 34082824

Save your precious samples
Our 384-well Human IL-8 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human/Monkey IL-8 Antibody Pair - BSA and Azide free ab241839
- Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) ab242515
- Anti-IL-8 antibody [EPR19358-108] - BSA and Azide free (Detector) ab242983

Related and recommended products
Human IL-8 ELISA Kit ab214030 is commonly used to measure IL-8 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:
- Human TNF alpha ELISA kit ab181421
- Human IL-6 ELISA kit ab178013
- Human IL-1 beta ELISA kit ab214025

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]
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Recovery

[ { "sample": "Cell culture media", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "84 - 94 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "83 - 90 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "84 - 92 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "82 - 91 %", "average": "= 87" }, { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational autoimmunity 8:100228 PubMed38225946

2024

ANKRD22 aggravates sepsis-induced ARDS and promotes pulmonary M1 macrophage polarization.

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Shi Zhang,Yao Liu,Xiao-Long Zhang,Yun Sun,Zhong-Hua Lu

The Journal of dermatology 50:1262-1271 PubMed37392066

2023

Protein inhibitor of activated STAT3 (PIAS3) attenuates psoriasis and associated inflammation.

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Unspecified application

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Jing Zhou,Huiying Bian,Nan Wu

Frontiers in nutrition 10:1162934 PubMed37125026

2023

Inhibitory effect of fucoidan on TNF-α-induced inflammation in human retinal pigment epithelium cells.

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Sol Lee,Eun Jeoung Lee,Gyu Min Lee,Ji-Hyun Yun,Wonbeak Yoo

Journal of dental sciences 18:636-644 PubMed37021271

2023

Myeloid-derived growth factor regulates high glucose-mediated apoptosis of gingival fibroblasts and induce AKT pathway activation and nuclear factor κB pathway inhibition.

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Linlin Gao,Zhenqiang Li,Wenxiao Chang,Yanyan Liu,Nan Zhang

Cell death discovery 9:84 PubMed36890151

2023

Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarization.

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Liqin Ning,Ningyuan Ye,Bai Ye,Zhiwei Miao,Tingting Cao,Weimin Lu,Danhua Xu,Chang Tan,Yi Xu,Jing Yan

Molecules (Basel, Switzerland) 28: PubMed36838778

2023

Kinkéliba () Leaf Extract Alleviates Skin Inflammation: In Vitro and In Vivo Study.

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Shuting Hu,James E Simon,Mingfu Wang,Yizhen Wu,Yumin Huang,Qingli Wu

EMBO molecular medicine 15:e16373 PubMed36511116

2022

PGF facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR CXC chemokine expression.

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Yan Zhao,Yi Lei,Huying Ning,Yaqiang Zhang,Guilin Chen,Chenchen Wang,Qiangyou Wan,Shumin Guo,Qian Liu,Ruotian Xie,Yujuan Zhuo,Shuai Yan,Jing Zhao,Fengjiang Wei,Lu Wang,Xiaohong Wang,Weidong Li,Hua Yan,Ying Yu

Frontiers in microbiology 13:1070201 PubMed36519178

2022

Biofilm formation and inflammatory potential of A possible cause of orthopedic implant-associated infections.

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Species

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Mastaneh Afshar,Andreas Møllebjerg,Gabriel Antonio Minero,Jacqueline Hollensteiner,Anja Poehlein,Axel Himmelbach,Jeppe Lange,Rikke Louise Meyer,Holger Brüggemann

Polymers 14: PubMed36433125

2022

Preparation, Characterization, and Anti-Adhesive Activity of Sulfate Polysaccharide from against .

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Bao Le,Duy Thanh Do,Hien Minh Nguyen,Bich Hang Do,Huong Thuy Le

Nature methods 19:1449-1460 PubMed36280722

2022

Geometric engineering of organoid culture for enhanced organogenesis in a dish.

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Species

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Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh
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We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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