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AB46032

Human IL-8 ELISA Kit

4

(1 Review)

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(73 Publications)

Human IL-8 ELISA Kit is a sandwich ELISA designed to quantify Human IL-8 with a sensitivity of 12.3 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 31.25 - 1000 pg/mL
- Cited in over 60 publications

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

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Sandwich ELISA - Human IL-8 ELISA Kit (AB46032)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Kit (AB46032)

Representative Standard Curve using ab46032

Sandwich ELISA - Human IL-8 ELISA Kit (AB46032)
  • sELISA

Unknown

Sandwich ELISA - Human IL-8 ELISA Kit (AB46032)

IL-8 detected in supernatants from control cells (C) or cells stimulated for 24 hours with 50 ng x mL-1 of PMA (ab120297) (P), and with the addition of 1 ug x mL-1 of LPS (Sigma) (P/L) for the last 6 hours. The positive control is included (Pos.). Results shown after background signal was subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 12.3 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

2h 15m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL-8 ELISA Kit ab46032 is a sandwich ELISA to measure Human IL-8 in serum, plasma, cell culture supernatant with a sensitivity of 12.3 pg/ml.

How the assay works

A monoclonal antibody specific for IL-8 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-8 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-8 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-8 present in the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human IL-8 ELISA Kit ab46032 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperature
3. Aspirate and wash each well
4. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
5. Aspirate and wash each well.
6. Add the TMB Solution to each well until color develops and then add the Stop Solution
7. Immediately begin recording the color development
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Precision

[ { "reproducibilityType": "Inter", "sample": "Pooled Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "Pooled Sample", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46032-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46032-CMP03" }, { "size":"4 x 1 Vial", "name":"IL-8 Standard", "number":"AB46032-CMP07" }, { "size":"4 x 1 Vial", "name":"IL-8 Control", "number":"AB46032-CMP05" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46032-CMP02" }, { "size":"2 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46032-CMP11" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46032-CMP08" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46032-CMP13" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46032-CMP01" }, { "size":"2 x 1 Unit", "name":"IL-8 Microplate (12 x 8 well strips)", "number":"AB46032-CMP06" }, { "size":"4 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46032-CMP12" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46032-CMP10" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46032-CMP09" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-IL-8", "number":"AB46032-CMP04" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46032-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46032-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-8 Control", "number":"AB46032-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-8 Microplate (12 x 8 well strips)", "number":"AB46032-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46032-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"IL-8 Standard", "number":"AB46032-CMP07", "productcode":"" }, { "size":"1 x 7 mL", "name":"Standard Diluent (Serum)", "number":"AB46032-CMP11", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46032-CMP08", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46032-CMP01", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-IL-8", "number":"AB46032-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46032-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46032-CMP09", "productcode":"" }, { "size":"2 x 1 Unit", "name":"Plastic Plate Covers", "number":"AB46032-CMP12", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46032-CMP13", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.
Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.
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Product protocols

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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8
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Publications (73)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40324823

2025

SLC38A9 is directly involved in Tat-induced endolysosome dysfunction and senescence in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Neda Rezagholizadeh,Gaurav Datta,Wendie A Hasler,Erica C Nguon,Elise V Smokey,Nabab Khan,Xuesong Chen

Molecular medicine reports 29: PubMed38639190

2024

Deciphering microglial activation and neuronal apoptosis post‑traumatic brain injury: The role of TYROBP in inflammation regulation networks.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Zhou,Huiping Song,Jingjing He,Wei Han,Qin Li

PloS one 19:e0295837 PubMed38335214

2024

Poly-(ADP-ribose) polymerases inhibition by olaparib attenuates activities of the NLRP3 inflammasome and of NF-κB in THP-1 monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Khamis Mustafa,Ying Han,Dan He,Ying Wang,Nan Niu,Pedro A Jose,Yinong Jiang,Jeffrey B Kopp,Hewang Lee,Peng Qu

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 40:34-44 PubMed37356505

2023

Human Rotator Cuff Tears Reveal an Age-Dependent Increase in Markers of Cellular Senescence and Selective Removal of Senescent Cells With Dasatinib + Quercetin Increases Genetic Expression of COL1A1 In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin C Hawthorne,Ian J Wellington,Joshua T Sabitsky,Kyle V Murphy,Owen P Karsmarski,Rohin O Thomas,Matthew R LeVasseur,Michael R Mancini,Maxwell T Trudeau,Sagar Gulati,Mary Beth R McCarthy,Mark P Cote,Ming Xu,Augustus D Mazzocca

BMC medicine 21:165 PubMed37118698

2023

Gut taste receptor type 1 member 3 is an intrinsic regulator of Western diet-induced intestinal inflammation.

Applications

Unspecified application

Species

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Woo-Jeong Shon,Jae Won Song,Seung Hoon Oh,Keon-Hee Lee,Hobin Seong,Hyun Ju You,Je Kyung Seong,Dong-Mi Shin

Cells 12: PubMed36899865

2023

Differential Effects of Nonsteroidal Anti-Inflammatory Drugs in an In Vitro Model of Human Leaky Gut.

Applications

Unspecified application

Species

Unspecified reactive species

Michele d'Angelo,Laura Brandolini,Mariano Catanesi,Vanessa Castelli,Cristina Giorgio,Margherita Alfonsetti,Mara Tomassetti,Mara Zippoli,Elisabetta Benedetti,Maria Candida Cesta,Sandro Colagioia,Pasquale Cocchiaro,Annamaria Cimini,Marcello Allegretti

Mediators of inflammation 2022:4258742 PubMed36405992

2022

ST3GAL3 Promotes the Inflammatory Response of Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Activating the TLR9/MyD88 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Xu,Xuegang Niu,Yifan Liu,Lining Liu

Annals of translational medicine 10:980 PubMed36267759

2022

Inhibitory effects of isoliquiritin on an atopic dermatitis model through the CD177/JAK2/STAT pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wu,Xiumei Mo,Ying Lin,Junfeng Liu,Siqi Ye,Yu Zhang,Xingxing Fan,Dacan Chen,Fenggen Yan

Allergologia et immunopathologia 50:147-154 PubMed35789415

2022

RAB11A aggravates PDGF-BB-stimulated proliferation, migration, and inflammation of airway smooth muscle cells via affecting the NF-κB and PI3K/AKT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Gong,Yunhai Hu,Jianqiong Huang,Haibo Wang

Antioxidants (Basel, Switzerland) 11: PubMed35624854

2022

Antioxidant Activities and Mechanisms of Tomentosin in Human Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Seyoung Yang,See-Hyoung Park,Sae Woong Oh,Kitae Kwon,Eunbi Yu,Chae Won Lee,Youn Kyoung Son,Changmu Kim,Byoung-Hee Lee,Jae Youl Cho,Youn-Jung Kim,Jongsung Lee
View all publications
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