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AB325443

Human IL-8 ELISA Kit, Chemiluminescent

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Human IL-8 ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human IL-8 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :1.3 - 940 pg/mL with a sensitivity of 0.47 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

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Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations. Undiluted samples are 100% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Standard curve comparison between the Chemiluminescent Human IL-8 SimpleStep ELISA and the colorimetric Human IL-8 SimpleStep ELISA (ab214030) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Dynamic range comparison between the Chemiluminescent Human IL-8 SimpleStep ELISA, colorimetric Human IL-8 SimpleStep ELISA (ab214030), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity with a shorter assay time (55 min vs 5 hr for the leading competitor).

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Serum of ten individual healthy human male donors and one individual with Alzheimer’s disease (AD) were measured in duplicate. Interpolated values are plotted (mean +/- SD, n=2). For the ten donors, the mean IL-8 concentration was determined to be 30.6 pg/mL with a range of 2.2 – 127.6 pg/mL. The mean IL-8 concentration measured in Alzheimer’s serum was determined to be 4.6 pg/mL.

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)
  • sELISA

Lab

Sandwich ELISA - Human IL-8 ELISA Kit, Chemiluminescent (AB325443)

Interpolated concentration of native IL-8 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 400 unstimulated and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.47 pg/mL

Range

1.3 - 940 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IL-8 ELISA Kit, Chemiluminescent (ab325443) is a rapid single-wash sandwich 55-min ELISA to measure Human IL-8 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Cerebrospinal Fluid with a sensitivity of 0.47 pg/ml.

How the assay works

Human IL-8 ELISA Kit, Chemiluminescent (ab325443) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human IL-8 (ab325443) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-8 antibody [EPR19358-37] BSA and Azide free (Capture) ab242515

- Anti-IL-8 antibody [EPR19358-108] BSA and Azide free (Detector) ab242983

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.8" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.5" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 103 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "84 - 92 %", "average": "= 88" }, { "sample": "EDTA Plasma", "range": "83 - 90 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "82 - 91 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Cerebral Spinal Fluid", "range": "84 - 94 %", "average": "= 89" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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