AB48481

Human IL-8 ELISA Set (without plates)

Name: Human IL-8 ELISA Set (without plates) (ab48481) | Abcam
Brand: Abcam Limited
SKU: AB48481
Price: 865 USD
Availability: InStock

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(3 Publications)

Human IL-8 ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Human IL-8 ELISA Set (without plates) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

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Sandwich ELISA - Human IL-8 ELISA Set (without plates) (AB48481)

sELISA

Supplier Data

Sandwich ELISA - Human IL-8 ELISA Set (without plates) (AB48481)

Human IL-8 ELISA Set. IL-8 Standard curve ranging from 62.5 to 2000 pg/mL

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 12.3 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

2h 15m

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's IL-8 Human in vitro ELISA Set is designed for the quantitative measurement of IL-8 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

The IL-8 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

This ELISA set is intended to measure human IL-8 in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

Sensitivity: < 29 pg / ml

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.

Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

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Product protocols

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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).

See full target information CXCL8

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncogene 41:2372-2389 PubMed35264742

2022

Cancer-associated fibroblast-induced lncRNA UPK1A-AS1 confers platinum resistance in pancreatic cancer via efficient double-strand break repair.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhang,Shangyou Zheng,Chonghui Hu,Guolin Li,Hongcao Lin,Renpeng Xia,Yuancheng Ye,Rihua He,Zhihua Li,Qing Lin,Rufu Chen,Quanbo Zhou

Analytica chimica acta 1011:28-34 PubMed29475482

2018

Ultrasensitive determination of receptor tyrosine kinase with a label-free electrochemical immunosensor using graphene quantum dots-modified screen-printed electrodes.

Applications

Unspecified application

Species

Unspecified reactive species

Fariba Mollarasouli,Verónica Serafín,Susana Campuzano,Paloma Yáñez-Sedeño,José M Pingarrón,Karim Asadpour-Zeynali

Evidence-based complementary and alternative medic 2014:131604 PubMed24812565

2014

Protection Effect of Zhen-Wu-Tang on Adriamycin-Induced Nephrotic Syndrome via Inhibiting Oxidative Lesions and Inflammation Damage.

Applications

ELISA

Species

Unspecified reactive species

Chun-Ling Liang,Jun-Biao Wu,Jie-Mei Lai,Shu-Fang Ye,Jin Lin,Hui Ouyang,Janis Ya-Xian Zhan,Jiu-Yao Zhou

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human IL-8 ELISA Set (without plates)

Sandwich ELISA - Human IL-8 ELISA Set (without plates) (AB48481)

Key facts

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Secondary Antibodies

Product promise