AB260072

Human IL1R1 ELISA Kit

Recombinant
SimpleStep
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Human IL1R1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL1R1 with a sensitivity of 99.56 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD121a, IL1R, IL1RA, IL1RT1, IL1R1, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, p80, IL-1R-alpha

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Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

sELISA

Supplier Data

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R1 concentration was determined to be 22 ng/mL with a range of 12 – 41 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Interpolated concentrations of native IL1R1 in human serum and plasma samples.

The concentrations of IL1R1 were measured in duplicate, interpolated from the IL1R1 standard curves, and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 12.5% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R1 concentration was determined to be 20 ng/mL in serum, 16 ng/mL in plasma (citrate), 14 ng/mL in plasma (EDTA), and 15 ng/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Interpolated concentrations of spike IL1R1 in human cell culture media samples.

The concentrations of IL1R1 were measured in duplicates, interpolated from the IL1R1 standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Example of human IL1R1 standard curve in Sample Diluent NS.

The IL1R1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 99.56 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IL1R1 ELISA Kit (ab260072) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL1R1 protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL1R1 with 99.56 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-1R1, Interleukin 1 receptor, type I, is a receptor for interleukin 1 alpha, beta, and their agonist. This enzyme is critical in the regulation of pro-inflammatory responses in response to tissue damage. Upon binding of its ligand, IL-1R1 dimerizes with IRAK-1 and 2 which leads to phosphorylation cascades and eventually transcription of anti-inflammatory genes. This kit targets the extracellular domain of IL-1R1.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" } ]

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Recovery

[ { "sample": "Serum", "range": "103 - 110 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "116 - 119 %", "average": "= 117" }, { "sample": "Heparin Plasma", "range": "111 - 119 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "111 - 115 %", "average": "= 113" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IL-1 Receptor I also known as IL-1R1 is a transmembrane protein with alternative names such as IL-1 receptor and IL-1 type I receptor. It acts as an important receptor for the pro-inflammatory cytokine Interleukin-1 (IL-1) family primarily IL-1α and IL-1β. IL-1R1 has a mass of approximately 80 kilodaltons and is expressed in a variety of cell types including endothelial cells epithelial cells and fibroblasts. It is also present on immune cells like monocytes and macrophages making it widespread in a vast range of tissues.

Biological function summary

IL-1R1 is a critical player in the initiation of immune responses serving as a component of the IL-1 signaling complex. When IL-1 binds to IL-1R1 it recruits the IL-1 receptor accessory protein (IL-1RAcP) to form a signaling complex that triggers downstream pathways. This interaction initiates a signaling cascade that activates nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) promoting the expression of inflammatory genes and mediating immune and inflammatory responses.

Pathways

IL-1R1 is central to the inflammatory response and immune signaling pathways. The IL-1 receptor complex facilitates the activation of NF-κB which plays a role in the body's response to stress cytokines and pathogens. The receptor acts in close relation with other proteins like IL-1RAcP and IRAKs (Interleukin-1 receptor-associated kinases) in these pathways creating a robust immune signaling network that drives the inflammatory response.

IL-1R1 is closely associated with inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease. Increased IL-1R1 expression and signaling contribute to chronic inflammation in these diseases. The interaction of IL-1β with IL-1R1 and subsequently with proteins like NF-κB leads to the persistence of inflammatory signals. Moreover targeting IL-1R1 and related proteins offers potential therapeutic strategies in mitigating the symptoms and progression of inflammatory disorders.

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Product protocols

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Target data

Receptor for IL1A, IL1B and IL1RN (PubMed : 2950091, PubMed : 37315560). After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (PubMed : 10653850).

See full target information IL1R1

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human IL1R1 ELISA Kit

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Sandwich ELISA - Human IL1R1 ELISA Kit (AB260072)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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