Human IL1R2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL1R2 with a sensitivity of 24.96 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 24.96 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Alternative names
CD121b, IL1RB, IL1R2, Interleukin-1 receptor type 2, IL-1R-2, IL-1RT-2, IL-1RT2, CD121 antigen-like family member B, CDw121b, IL-1 type II receptor, Interleukin-1 receptor beta, Interleukin-1 receptor type II, IL-1R-beta
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Human IL1R2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL1R2 with a sensitivity of 24.96 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 62.5 - 4000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 24.96 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 1.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 2.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 110 | Range 106 - 116 % |
Sample type EDTA Plasma | Average % = 110 | Range 105 - 118 % |
Sample type Urine | Average % = 95 | Range 93 - 99 % |
Sample type Heparin Plasma | Average % = 110 | Range 103 - 118 % |
Sample type Citrate plasma | Average % = 107 | Range 104 - 113 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IL1R2 ELISA Kit (ab269548) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL1R2 protein in edta plasma, serum, urine, cit plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL1R2 with 24.96 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
IL1R2, interleukin 1 receptor, type II, is a key protein involved in the negative regulation of inflammatory and immune signaling. IL1R2 inhibits interleukins 1a and 1b and IL-1RAcp by binding to them without propagating a signaling cascade. Given its pivotal role in cell signaling, IL1R2 is currently under investigation for its role in a host of inflammatory and autoimmune-related pathologies. The antibodies for this kit were raised against a recombinant protein fragment of the mature human protein. This protein does not share a high sequence identity with mouse or rat and does not react. However, this kit does cross-react with monkey serum.
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5 product images
Sandwich ELISA - Human IL1R2 ELISA Kit (ab269548)
Example of human IL1R2 standard curve in Sample Diluent NS.
The IL1R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IL1R2 ELISA Kit (ab269548)
Interpolated concentrations of native IL1R2 in human serum and plasma samples.
The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20, plasma (citrate) 1:20, plasma (EDTA) 1:20, and plasma (heparin) 1:20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 40.50 ng/mL in serum, 24.84 ng/mL in plasma (citrate), 30.33 ng/mL in plasma (EDTA), and 31.03 ng/mL in plasma (heparin).
Sandwich ELISA - Human IL1R2 ELISA Kit (ab269548)
Interpolated concentrations of native IL1R2 in human urine samples.
The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 2.87 ng/mL in urine.
Sandwich ELISA - Human IL1R2 ELISA Kit (ab269548)
Interpolated concentrations of native IL1R2 in human cell culture supernatant samples.
The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 100% and unstimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 226 pg/mL in stimulated PBMC cell culture supernatant and was undetectable in unstimulated PBMC cell culture supernatant.
Sandwich ELISA - Human IL1R2 ELISA Kit (ab269548)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 43 ng/mL with a range of 31 – 77 ng/mL.
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