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AB314736

Human IL27RA/WSX-1 ELISA Kit

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Human IL27RA/WSX-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL27RA/WSX-1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CRL1, TCCR, WSX1, UNQ296/PRO336, IL27RA, Interleukin-27 receptor subunit alpha, IL-27 receptor subunit alpha, IL-27R subunit alpha, IL-27R-alpha, IL-27RA, Cytokine receptor WSX-1, Cytokine receptor-like 1, Type I T-cell cytokine receptor, ZcytoR1

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Sandwich ELISA - Human IL27RA/WSX-1 ELISA Kit (AB314736)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL27RA/WSX-1 ELISA Kit (AB314736)

Example of standard curve using ab314736. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.85 ng/mL

Range

0.85 - 200 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL27RA/WSX-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL27RA/WSX-1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human IL27RA/WSX-1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "124 - 133 %", "average": "= 128.3" }, { "sample": "Plasma", "range": "69 - 90 %", "average": "= 76.42" }, { "sample": "Cell culture media", "range": "132 - 140 %", "average": "= 135.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314736-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314736-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human WSX-1 Protein Standard", "number":"AB314736-CMP10", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314736-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human WSX-1 Detection Antibody", "number":"AB314736-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314736-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human WSX-1 Antibody-coated ELISA Plate", "number":"AB314736-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314736-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin", "number":"AB314736-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"96-Well Plate Sealing Film", "number":"AB314736-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The WSX-1 protein also known as IL-27Rα or TCCR is a subunit of the interleukin-27 receptor. It weighs approximately 74 kDa and is mainly expressed on various immune cells including T cells B cells and monocytes. WSX-1 engages in the immune signaling by interacting specifically with its ligand IL-27 to drive immunological responses. This receptor belongs to the type I cytokine receptor family and is structurally characterized by certain conserved motifs important for its signaling functions.
Biological function summary

WSX-1 plays a role in regulating immune cell activity and differentiation. It acts as a part of a receptor complex with gp130 to form the IL-27 receptor which transmits signals that modulate the activity of T helper cells. This signaling influences the production of pro-inflammatory and anti-inflammatory cytokines impacting immune system balance. The function of WSX-1 remains critical for controlling inflammatory responses and ensuring immune tolerance.

Pathways

WSX-1 contributes to the JAK/STAT signaling pathway and influences the differentiation of Th1 cells. The connection to JAK/STAT signaling involves various proteins such as STAT1 and STAT3 which transduce IL-27-induced signals leading to gene expression changes in immune cells. Through its action in these pathways WSX-1 regulates immune responses and can influence inflammation dynamics in various biological contexts.

WSX-1 has associations with autoimmune diseases and infectious conditions. Alterations in its signaling pathways can lead to dysregulated immune responses contributing to diseases like multiple sclerosis and inflammatory bowel disease. The protein’s interaction with IL-27 is central in these conditions as it influences the pro-inflammatory and regulatory functions of the immune system. Furthermore studies suggest a link between WSX-1 activity and the modulation of responses against infections linking it to potential therapeutic targets for managing related disorders.
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Product protocols

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Target data

Receptor for IL27. Requires IL6ST/GP130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL6ST/GP130 and CNTFR (PubMed : 19386761). Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
See full target information IL27RA
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB281998

Anti-IL27RA/WSX-1 antibody [EPR24560-54]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-IL27RA/WSX-1 antibody [EPR24560-54] (AB281998)

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