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AB267599

Human IL36RN ELISA kit

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Human IL36RN ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human IL36RN in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

FIL1D, IL1F5, IL1HY1, IL1L1, IL1RP3, UNQ1896/PRO4342, IL36RN, Interleukin-36 receptor antagonist protein, IL-36Ra, FIL1 delta, IL-1-related protein 3, Interleukin-1 HY1, Interleukin-1 delta, Interleukin-1 family member 5, Interleukin-1 receptor antagonist homolog 1, Interleukin-1-like protein 1, IL-1RP3, IL-1HY1, IL-1 delta, IL-1F5, IL-1ra homolog 1, IL-1L1

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Sandwich ELISA - Human IL36RN ELISA kit (AB267599)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL36RN ELISA kit (AB267599)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.07 ng/mL

Range

0.069 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IL36RN ELISA Kit is designed for the quantitative determination of IL36RN in cell culture supernatants, plasma and serum samples.

Δ Note: Human IL36RN concentration is low in normal serum/plasma and may not be detectable in this assay.

This assay employs an antibody specific for human IL36RN coated on a 96-well plate. Standards and samples are pipetted into the wells and IL36RN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL36RN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL36RN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit shows no cross-reactivity with the following cytokines tested: Human IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 RA (IL-1 F3), IL-18 (IL-1 F4), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-38 (IL-1 F10), IL-33 (IL-1 F11).

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "135 - 150 %", "average": "= 139.9" }, { "sample": "Plasma", "range": "111 - 145 %", "average": "= 129.1" }, { "sample": "Cell culture media", "range": "115 - 142 %", "average": "= 134.6" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267599-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267599-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267599-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human IL36RN coated Microplate (12 x 8 wells)", "number":"AB267599-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IL36RN Standard (Lyophilized)", "number":"AB267599-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267599-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267599-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267599-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human IL36RN Detection Antibody", "number":"AB267599-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.
See full target information IL36RN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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