Human ILT-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ILT-3 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.469 - 350 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibitory receptor involved in the down-regulation of the immune response and the development of immune tolerance (PubMed:11875462). Receptor for FN1 (PubMed:34089617). Receptor for apolipoprotein APOE (PubMed:30333625). Receptor for ALCAM/CD166 (PubMed:29263213). Inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:9151699). Inhibits FCGR1A/CD64-mediated monocyte activation by inducing phosphatase-mediated down-regulation of the phosphorylation of multiple proteins including LCK, SYK, LAT and ERK, leading to a reduction in TNF production (PubMed:19833736). This inhibition of monocyte activation occurs at least in part via binding to FN1 (PubMed:34089617). Inhibits T cell proliferation, inducing anergy, suppressing the differentiation of IFNG-producing CD8+ cytoxic T cells and enhancing the generation of CD8+ T suppressor cells (PubMed:16493035, PubMed:19833736, PubMed:29263213). Induces up-regulation of CD86 on dendritic cells (PubMed:19860908). Interferes with TNFRSF5-signaling and NF-kappa-B up-regulation (PubMed:11875462).
Alternative names
CD85k, ILT3, LIR5, LILRB4, Leukocyte immunoglobulin-like receptor subfamily B member 4, B4, CD85 antigen-like family member K, Immunoglobulin-like transcript 3, Leukocyte immunoglobulin-like receptor 5, Monocyte inhibitory receptor HM18, ILT-3, LIR-5
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Human ILT-3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ILT-3 with a sensitivity of 2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.469 - 350 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2 pg/mL
- General recovery
- 105%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 7.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 105 | Range 101 - 111 % |
Sample type Citrate plasma | Average % = 92 | Range 82 - 99 % |
Sample type EDTA Plasma | Average % = 99 | Range 90 - 108 % |
Sample type Heparin Plasma | Average % = 103 | Range 100 - 107 % |
Sample type Cell culture supernatant | Average % = 101 | Range 98 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ILT-3 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ILT-3 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human ILT-3 with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ILT-3 also known as Immunoglobulin-like transcript 3 is a protein predominantly expressed on dendritic cells and monocytes. It is a type I transmembrane protein belonging to the leukocyte immunoglobulin-like receptor family. The ILT-3 protein has a molecular mass of approximately 60 kDa. In addition to its presence on dendritic cells and monocytes ILT-3 can be detected in low levels on some types of macrophages. It plays a regulatory role in the immune system which can influence how the body responds to pathogens and tissue damage.
- Biological function summary
ILT-3 acts as an inhibitory receptor that modulates immune responses. It is often linked with the control of T cell activation and the maintenance of immune tolerance. ILT-3 does not form part of a larger protein complex. Its inhibitory function helps in preventing overactivation of the immune system by modulating the signaling pathways necessary for immune response. This modulation occurs through ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) domains that recruit phosphatases helping in dephosphorylating key signaling proteins and aiding in downregulation of immune cell activities.
- Pathways
The ILT-3 protein is involved in important immune regulatory pathways contributing significantly to the immune checkpoint pathway. It interacts with other immunoglobulin-like receptors and is known to influence pathways involving PD-1 (Programmed cell death protein 1) and CTLA-4 (Cytotoxic T-lymphocyte-associated protein 4). By affecting these pathways ILT-3 serves as a checkpoint that ensures immune homeostasis prevents excess inflammation and influences the balance between immune activation and suppression within the body.
- Associated diseases and disorders
ILT-3 has been implicated in autoimmune diseases and transplant rejection. It helps in suppressing immune responses that can lead to conditions like rheumatoid arthritis and could negatively affect organ transplant outcomes by minimizing the chance of rejection. ILT-3's role in these conditions connects it to proteins such as HLA-G and PD-L1 which are also involved in immune modulation and contribute to disease pathogenesis. Understanding ILT-3’s role provides insights into potential therapeutic targets for these diseases.
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6 product images
Sandwich ELISA - Human ILT-3 ELISA Kit (ab320789)
Example of human ILT-3 standard curve in Sample Diluent NS. Example of human ILT-3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ILT-3 ELISA Kit (ab320789)
Interpolated concentrations of human ILT-3 in in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and THP-1 cell culture supernatant. Interpolated concentration of native ILT-3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 25%, plasma (heparin) 25%, and THP-1 cell culture supernatant 8%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ILT-3 ELISA Kit (ab320789)
Example of human ILT-3 standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human ILT-3 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ILT-3 ELISA Kit (ab320789)
Interpolated concentrations of human ILT-3 in serum in 96-well vs. 384-well plates. Interpolated concentration of native ILT-3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ILT-3 ELISA Kit (ab320789)
Cross reactivity of related LILR family members. 50 ng/mL of recombinant human LILRA5 was tested for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human ILT-3 (LILRB4) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No cross reactivity was observed.
Sandwich ELISA - Human ILT-3 ELISA Kit (ab320789)
Interference testing of related LILR family members. 250 pg/mL of recombinant human LILRA5 was tested interference with 125 pg/ml of recombinant human ILT-3. Percent reactivity was determined by interpolating the protein concentration from the human ILT-3 (LILRB4) standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested. No significant interference was observed.
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