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AB108743

Human Anti-Influenza virus A IgA ELISA Kit

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(2 Publications)

Human Anti-Influenza virus A IgA ELISA Kit is an indirect ELISA for the qualitative detection of IgA class antibodies against Influenza virus A in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Influenza virus A IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Influenza virus A in Human serum and plasma.

A 96-well plate has been precoated with Influenza virus A antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgA sample captured in plate.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 19, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108743-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Influenza Virus A IgA Negative Control", "number":"AB108743-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"Influenza Virus A IgA Positive Control", "number":"AB108743-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108743-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Influenza virus A (IgA) Coated Microplate (12 x 8 wells)", "number":"AB108743-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108743-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108743-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108743-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"Influenza Virus A IgA Cut-off Control", "number":"AB108743-CMP09", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgA Sample Diluent", "number":"AB108743-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Influenza virus A anti-IgA HRP Conjugate", "number":"AB108743-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108743-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza Virus A IgA is an immunoglobulin that specifically recognizes and binds to the influenza virus particularly the Influenza A virus which includes subtypes like H1N1. This immunoglobulin plays an important role in the immune response by preventing the virus from invading mucosal tissues. It weighs approximately 150 kDa and is mainly expressed along mucosal surfaces such as the respiratory and gastrointestinal tracts. Also referred to as can IgA HA and IgA pos it serves as an important indicator in evaluating immune protection against the Influenza A virus.
Biological function summary

Immunoglobulin A (IgA) plays a critical role in the immune defense mechanisms against mucosal pathogens. It is a part of the mucosal immunological complex and contributes to the first line of defense. IgA is the most abundant type of immunoglobulin present in mucosal areas and assists in neutralizing viruses and preventing infection. It binds to the HA proteins on the viral surface blocking entry into host cells.

Pathways

Influenza virus A IgA is important in the mucosal immune response pathway. It interacts with other components of the immune system such as secretory component/SC and polymeric Ig receptor/pIgR which facilitate the transport of IgA across epithelial cells to mucosal surfaces. These interactions help form barriers against pathogens and participate in pathways like receptor-mediated endocytosis and transport across epithelial barriers.

Influenza virus A IgA is important for understanding influenza infection and related respiratory diseases. The presence and levels of this immunoglobulin identified through IgA ELISA tests are correlated with protection against influenza. It interacts closely with HA proteins of the influenza virus and its absence or insufficient levels may result in increased susceptibility to the influenza virus leading to severe respiratory illness.
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 10:1116634 PubMed37168053

2023

Healthy adults supplemented with a nutraceutical formulation containing gel, rosemary and enhances the effect of influenza vaccination in a randomized, triple-blind, placebo-controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Erin D Lewis,David C Crowley,Najla Guthrie,Malkanthi Evans

Vaccines 10: PubMed35891309

2022

Adults with Trisomy 21 Have Differential Antibody Responses to Influenza A.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie James,Robert C Haight,Cassandra Hanna,Lindsey Furton
View all publications
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websiteProtocolBooklet
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